Human Siglec-10 Antibody

Catalog # Availability Size / Price Qty
AF2130
AF2130-SP
Human Siglec-10 Antibody in Data
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Product Details
Citations (2)
FAQs
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Reviews (2)

Human Siglec-10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Siglec‑10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) Siglec-5 is observed and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-3, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Siglec-10
Met17-Thr546
Accession # Q96LC7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Siglec‑10 Fc Chimera (Catalog # 2130-SL)
Flow Cytometry
2.5 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1.5 μg/mL of biotinylated 6’‑Sialyllactose-Polyacrylamide to immobilized Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) coated at 5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Siglec-10 antibody in Human B Cells antibody by Flow Cytometry. View Larger

Detection of Siglec‑10 in Human B Cells by Flow Cytometry. Human whole blood CD19+B cells were stained with Goat Anti-Human Siglec-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2130, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phy-coerythrin-conjugated Anti-Goat IgG Secondary Anti-body (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-10

Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).

References
  1. Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431.
  2. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609.
  3. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  6. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900.
  7. Li, N. et al. (2001) J. Biol. Chem. 276:28106.
  8. Munday, J. et al. (2001) Biochem. J. 355:489.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
  10. Aizawa, H. et al. (2003) Genomics 82:521.
  11. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
Long Name
Sialic Acid Binding Ig-like Lectin 10
Entrez Gene IDs
89790 (Human)
Alternate Names
MGC126774; PRO940; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; Siglec10; Siglec-10; siglec-like gene 2; Siglec-like protein 2; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7

Product Datasheets

Citations for Human Siglec-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Preferential use of Siglec-1 or Siglec-10 by type 1 and type 2 PRRSV strains to infect PK15S1-CD163 and PK15S10-CD163 cells
    Authors: J Xie, I Christiaen, B Yang, I Trus, B Devriendt, T Cui, R Wei, HJ Nauwynck
    Vet. Res., 2018;49(1):67.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  2. Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells.
    Authors: Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS
    Allergy, 2006;61(6):769-76.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Siglec-10 Antibody
By Anonymous on 11/04/2020
Application: ICC/IF Sample Tested: Melanoma tissue Species: Human

Human Siglec-10 Antibody
By Anonymous on 03/13/2020
Application: B/N Sample Tested: NK cell Species: Human