Human SIRP beta 2 Antibody (1008702)

R&D Systems | Catalog # MAB99981

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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1008702
View all SIRP beta 2 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line, HEK293 derived Human SIRP beta 2
Gln33-Gly287
Accession # Q5JXA9

Specificity

Detects human SIRP beta 2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human SIRP beta 2 Antibody (1008702)

Detection of SIRP beta 2 antibody in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP antibody by Flow Cytometry.

Detection of SIRP beta 2 in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with (A) SIRP beta 2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB99981) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
Detection of SIRP beta 2 antibody in THP-1 Cells antibody by Flow Cytometry.

Detection of SIRP beta 2 in THP-1 Cells by Flow Cytometry.

THP-1 monocytic leukemia cell line was stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB99981, filled histogram) or Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human SIRP beta 2 Antibody (1008702)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP and THP-1 Monocytic Leukemia Cell Line

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

References

  1. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
  3. Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
  4. Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
  5. Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
  6. Veillette A. (2018) Trends Immunol. 39:173.

Long Name

Signal-regulatory Protein beta 2

Alternate Names

PTPN1L, PTPNS1L3

Entrez Gene IDs

284759 (Human)

Gene Symbol

SIRPB2

UniProt

Q5JXA9

Additional SIRP beta 2 Products

Product Documents for Human SIRP beta 2 Antibody (1008702)

Certificate of Analysis

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Product Specific Notices for Human SIRP beta 2 Antibody (1008702)

For research use only

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