Human SLAM/CD150 Antibody

Catalog # Availability Size / Price Qty
MAB16421-100
MAB16421-SP
Human SLAM/CD150 Antibody in ELISA
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Human SLAM/CD150 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SLAM/CD150 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 123303
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150
Ala21-Pro237
Accession # Q13291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SLAM/CD150 DuoSet ELISA Kit (Catalog # DY164) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human SLAM/CD150 ELISA Standard Curve. Recombinant Human SLAM/CD150 protein was serially diluted 2-fold and captured by Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB1642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SLAM/CD150 Monoclonal Antibody (Catalog # MAB16421) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLAM/CD150

Signaling lymphocytic activation molecule (SLAM, SLAMF1; CD150) was the first identified of a family of type I transmembrane (TM) lymphocyte activating receptors. SLAM homotypic adhesion bidirectionally stimulates T and B cells. SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. The 70 kDa glycoprotein contains a 216 amino acid (aa) extracellular domain (ECD) with one C2 type and one V type Ig-like domain, a 20 aa TM sequence and a 76 aa SH2-binding cytoplasmic domain. One splice variant has a shorter cytoplasmic tail and another lacks the TM sequence and is secreted. Human and mouse SLAM ECD share 60% aa identity.

Long Name
Signaling Lymphocytic Activation Molecule
Entrez Gene IDs
6504 (Human); 27218 (Mouse)
Alternate Names
CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAM; SLAMCD150 antigen; SLAMF1

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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