Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Flow Cytometry, COMET

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 640438
View all SLC22A2/OCT2 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human SLC-22A2

Accession # O15244

Specificity

Detects human SLC22A2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human SLC22A2/OCT2 Antibody

Detection of SLC22A2/OCT2 in Human Kidney via seqIF™ staining on COMET™​

SLC22A2/OCT2 was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human SLC22A2/OCT2, Monoclonal Antibody (Catalog # MAB6547) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​
SLC22A2 antibody in Human Kidney by Immunohistochemistry (IHC-P).

SLC22A2 in Human Kidney.

SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of SLC22A2/OCT2 in HEK293 Human Cell Line Transfected with Human SLC22A2/OCT2 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with EGP and (A) human SLC22A2/OCT2 or (B) irrelevant protein was stained with Mouse Anti-Human SLC22A2/OCT2 Monoclonal Antibody (Catalog # MAB6547) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human SLC22A2/OCT2 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human SLC22A2/OCT2 and eGFP

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Multiplex Immunofluorescence

20 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLC22A2/OCT2

Solute carrier family 22 member 2 (SLC22A2; also hOCT2) is a 65 kDa member of the major facilitator superfamily and organic cation transporter family of proteins.   Human SLC22A2 is synthesized as a multipass transmembrane protein that is 555 amino acids (aa) in length.  Human SLC22A2 contains one potential site for N-linked glycosylation.  There are also two additional isoforms for human SLC22A2.   Isoform 2 has a 57 aa substitution for aa 427-483 and a deletion of the 72 aa at positions 484-555.   Isoform 3 has an 18 aa substitution for aa 225-242 and a deletion of residues 243-555.  SLC22A2 has its highest expression in the kidney.  It is also expressed at lower levels in neurons of the cerebral cortex and in various subcortical nuclei.

Long Name

Organic Cation Transporter 2

Alternate Names

OCT2

Entrez Gene IDs

6582 (Human); 29503 (Rat)

Gene Symbol

SLC22A2

UniProt

O15244

Additional SLC22A2/OCT2 Products

Product Documents for Human SLC22A2/OCT2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SLC22A2/OCT2 Antibody

For research use only

Citations for Human SLC22A2/OCT2 Antibody

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Protocols

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