Human SLC22A2 Antibody Summary
Accession # O15244
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
SLC22A2 in Human Kidney. SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Solute carrier family 22 member 2 (SLC22A2; also hOCT2) is a 65 kDa member of the major facilitator superfamily and organic cation transporter family of proteins. Human SLC22A2 is synthesized as a multipass transmembrane protein that is 555 amino acids (aa) in length. Human SLC22A2 contains one potential site for N-linked glycosylation. There are also two additional isoforms for human SLC22A2. Isoform 2 has a 57 aa substitution for aa 427-483 and a deletion of the 72 aa at positions 484-555. Isoform 3 has an 18 aa substitution for aa 225-242 and a deletion of residues 243-555. SLC22A2 has its highest expression in the kidney. It is also expressed at lower levels in neurons of the cerebral cortex and in various subcortical nuclei.
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Cell and Tissue Staining Kits
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