Human SLC22A2 Antibody MAB6547: R&D Systems

Human SLC22A2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SLC22A2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 640438
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human SLC-22A2

    Accession # O15244
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
SLC22A2 in Human Kidney. SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLC22A2/OCT2
Solute carrier family 22 member 2 (SLC22A2; also hOCT2) is a 65 kDa member of the major facilitator superfamily and organic cation transporter family of proteins.   Human SLC22A2 is synthesized as a multipass transmembrane protein that is 555 amino acids (aa) in length.  Human SLC22A2 contains one potential site for N-linked glycosylation.  There are also two additional isoforms for human SLC22A2.   Isoform 2 has a 57 aa substitution for aa 427-483 and a deletion of the 72 aa at positions 484-555.   Isoform 3 has an 18 aa substitution for aa 225-242 and a deletion of residues 243-555.  SLC22A2 has its highest expression in the kidney.  It is also expressed at lower levels in neurons of the cerebral cortex and in various subcortical nuclei.
  • Long Name:
    Organic Cation Transporter 2
  • Entrez Gene IDs:
    6582 (Human); 29503 (Rat)
  • Alternate Names:
    hOCT2; MGC32628; OCT2; OCT2Organic cation transporter 2; SLC22A2; solute carrier family 22 (organic cation transporter), member 2; solute carrier family 22 member 2
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