Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 525122
View all SorLA Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SorLA
Ser82-Ser1550
Accession # Q92673

Specificity

Detects human SorLA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human SorLA Antibody

SorLA antibody in Human Brain by Immunohistochemistry (IHC-P).

SorLA in Human Brain.

SorLA was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human SorLA Monoclonal Antibody (Catalog # MAB5699) at 25 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasmic granules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
SorLA antibody in Human Brain by Immunohistochemistry (IHC-P).

SorLA in Human Brain.

SorLA was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human SorLA Monoclonal Antibody (Catalog # MAB5699) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human SorLA Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SorLA

SorLA (sortilin-related receptor, LDLR class A repeats-containing), also called LR11 or SORL1, is a 250 kDa type I transmembrane glycoprotein of the Sortilin family of Vps10p-domain receptors. SorLA is found in both intracellular and surface membranes in the central nervous system, and mediates trafficking of proteins such as the amyloid precursor protein (APP). The 2214 amino acid (aa) human SorLA includes a 28 aa signal sequence, a 53 aa furin-cleaved propeptide, and a 2056 aa extracellular domain. The region used as an immunogen, which includes BNR, LDLR class A and B, and EGF-like domains, but excludes fibronectin-like domains, shares 95% aa identity with mouse and rat SorLA.

Long Name

Sortilin-related Receptor Containing LDLR Class A Repeats

Alternate Names

gp250, LR11, LRP9, SORL1

Entrez Gene IDs

6653 (Human); 20660 (Mouse); 300652 (Rat)

Gene Symbol

SORL1

UniProt

Q92673

Additional SorLA Products

Product Documents for Human SorLA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SorLA Antibody

For research use only

Related Research Areas

Obesity

Citations for Human SorLA Antibody

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Protocols

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