Human SOX2 Biotinylated Antibody Summary
Accession # P48431
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
SOX2 in Human iPSK3 cells. SOX2 was detected in immersion fixed human plasmid-derived induced pluripotent stem cells (iPSK3) using Goat Anti-Human SOX2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-6).
- Graham, V. et al. (2003) Neuron 39:749.
- Avilion, A.A. et al. (2003) Genes Dev. 17:126.
- Kishi, M. et al. (2000) Development 127:791.
- Yuan, H. et al. (1995) Genes Dev. 9:2635.
- Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
- Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Citations for Human SOX2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Eva1 Maintains the Stem-like Character of Glioblastoma-Initiating Cells by Activating the Noncanonical NF-?B Signaling Pathway
Authors: N Ohtsu, Y Nakatani, D Yamashita, S Ohue, T Ohnishi, T Kondo
Cancer Res., 2016;76(1):171-81.
Sample Types: Whole Tissue
Conversion of differentiated cancer cells into cancer stem-like cells in a glioblastoma model after primary chemotherapy.
Authors: Auffinger B, Tobias A, Han Y, Lee G, Guo D, Dey M, Lesniak M, Ahmed A
Cell Death Differ, 2014;21(7):1119-31.
Sample Types: Whole Cells
Applications: Flow Cytometry
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