Human SREC-I/SCARF1 Antibody

Catalog # Availability Size / Price Qty
MAB2409
MAB2409-SP
Human SREC-I/SCARF1 Antibody in Flow Cytometry
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Human SREC-I/SCARF1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SREC‑I/SCARF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SREC-2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 373606
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human SREC‑I/SCARF1
Ser20-Thr421
Accession # Q14162
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human SREC-I/SCARF1 Fc Chimera (Catalog # 2409-SR)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SREC‑I/SCARF1 in HUVEC Human Cells by Flow Cytometry. View Larger

Detection of SREC‑I/SCARF1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human SREC-I/SCARF1 Monoclonal Antibody (Catalog # MAB2409, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SREC-I/SCARF1

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL(OxLDL) (1‑3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I; also called SCARF1) and SREC-2 are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain 8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain 8 plus domains 9 and 10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa and is expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region, human SREC-I shares 76% and 53% aa sequence identity with mouse SREC-I and human SREC-2, respectively.

References
  1. Horiuchi, S. et al. (2003) Amino Acids 25:283.
  2. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
  3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  4. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  5. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
  6. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
  7. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
  8. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.
Long Name
Scavenger Receptor Expressed by Endothelial Cells 1
Entrez Gene IDs
8578 (Human)
Alternate Names
Acetyl LDL receptor; KIAA0149endothelial cells scavenger receptor; MGC47738; SCARF1; scavenger receptor class F, member 1; Scavenger receptor expressed by endothelial cells 1; SREC1; SRECI; SREC-I; SRECscavenger receptor expressed by endothelial cells; SR-F1

Product Datasheets

Citation for Human SREC-I/SCARF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization.
    Authors: Baur S, Rautenberg M, Faulstich M, Grau T, Severin Y, Unger C, Hoffmann W, Rudel T, Autenrieth I, Weidenmaier C
    PLoS Pathog, 2014;10(5):e1004089.
    Species: Cotton Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Secondary Antibodies

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