Human ST2/IL-33R DuoSet ELISA

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Ancillary Products Available
Human ST2/IL-33R ELISA Standard Curve
2 Images
Product Details
Citations (22)
Supplemental Products
Reviews (3)

Human ST2/IL-33R DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
31.2 - 2,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ST2 / IL-33 R. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Examples

Human ST2/IL-33R ELISA Standard Curve

Human ST2 / IL-33 R ELISA Standard Curve

Product Datasheets

Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ST2/IL-33R

ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

Long Name:
Interleukin 33 Receptor
Entrez Gene IDs:
9173 (Human); 17082 (Mouse)
Alternate Names:
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed

Citations for Human ST2/IL-33R DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Early bilirubinemia after allogeneic stem cell transplantation-an endothelial complication
    Authors: H Dai, O Penack, A Radujkovic, D Schult, J Majer-Laut, IW Blau, L Bullinger, S Jiang, C Müller-Tid, P Dreger, T Luft
    Bone marrow transplantation, 2021;0(0):.
    Species: Human
    Sample Types: Serum
  2. Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases
    Authors: RM Torrente-R, CM Martín, M Gamella, M Pedrero, N Martínez-B, P Navarro, P García de, JM Pingarrón, S Campuzano
    Biosensors, 2021;11(6):.
    Species: Human
    Sample Types: Serum
  3. Prospective diagnostic accuracy study of plasma soluble ST2 for diagnosis of acute aortic syndromes
    Authors: F Morello, A Bartalucci, M Bironzo, M Santoro, E Pivetta, A Ianniello, F Rumbolo, G Mengozzi, E Lupia
    Sci Rep, 2020;10(1):3103.
    Species: Human
    Sample Types: Plasma
  4. Expression of the Novel Cardiac Biomarkers sST2, GDF-15, suPAR, and H-FABP in HFpEF Patients Compared to ICM, DCM, and Controls
    Authors: P Jirak, R Pistulli, M Lichtenaue, B Wernly, V Paar, LJ Motloch, R Rezar, C Jung, UC Hoppe, PC Schulze, D Kretzschma, RC Braun-Dull, T Bekfani
    J Clin Med, 2020;9(4):.
    Species: Human
    Sample Types: Serum
  5. EASIX for prediction of survival in lower-risk myelodysplastic syndromes
    Authors: A Merz, U Germing, G Kobbe, J Kaivers, A Jauch, A Radujkovic, M Hummel, A Benner, M Merz, P Dreger, T Luft
    Blood Cancer J, 2019;9(11):85.
    Species: Human
    Sample Types: Plasma
  6. Pre-transplant testosterone and outcome of men after allogeneic stem cell transplantation
    Authors: A Radujkovic, L Kordelas, J Krzykalla, A Benner, D Schult, J Majer-Laut, DW Beelen, C Müller-Tid, C Kasperk, P Dreger, T Luft
    Haematologica, 2019;0(0):.
    Species: Human
    Sample Types: Serum
  7. GDF-15 is a better complimentary marker for risk stratification of arrhythmic death in non-ischaemic, dilated cardiomyopathy than soluble ST2
    Authors: S Stojkovic, A Kaider, L Koller, M Brekalo, J Wojta, A Diedrich, S Demyanets, T Pezawas
    J. Cell. Mol. Med., 2018;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Magnitude of Soluble ST2 as a Novel Biomarker for Acute Aortic Dissection.
    Authors: Yuan Wang, Xin Tan, Hai Gao, Hui Yuan, Rong Hu, Lixin Jia, Junming Zhu, Lizhong Sun, Hongjia Zhang, Lianjun Huang, Dong Zhao, Pei Gao, Jie Du
    Circulation, 2018;0(0):1524-4539.
    Species: Human
    Sample Types: Plasma
  9. IL33 and IL1RL1 variants are associated with asthma and atopy in a Brazilian population
    Authors: GA Queiroz, RS Costa, NM Alcantara-, G Nunes de O, ML Barreto, VL Carneiro, CA Figueiredo
    Int. J. Immunogenet, 2017;44(2):51-61.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Predictive value of low interleukin-33 in critically ill patients
    Authors: KA Krychtiuk, S Stojkovic, M Lenz, M Brekalo, K Huber, J Wojta, G Heinz, S Demyanets, WS Speidl
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Serum
  11. A functional IL1RL1 variant regulates corticosteroid-induced sST2 expression in ulcerative colitis
    Authors: D Díaz-Jimén, L Núñez, M De la Fuen, K Dubois-Cam, H Sepúlveda, M Montecino, A Torres-Riq, P García-Gon, J Chnaiderma, A Vossenkamp, TT MacDonald, D Simian, MJ González, JA Cidlowski, R Quera, MA Hermoso
    Sci Rep, 2017;7(1):10180.
    Species: Human
    Sample Types: Serum
  12. Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
    Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
    Cytokine, 2017;0(0):.
    Species: Human
    Sample Types: Tissue Culture Supernates
  13. Soluble plasma proteins ST2 and CD163 as early biomarkers of nephropathy in Swedish patients with diabetes, 15-34�years of age: a prospective cohort study
    Authors: M Samuelsson, J Dereke, MK Svensson, M Landin-Ols, M Hillman, on the beh
    Diabetol Metab Syndr, 2017;9(0):41.
    Species: Human
    Sample Types: Plasma
  14. Aqueous two-phase system patterning of detection antibody solutions for cross-reaction-free multiplex ELISA.
    Authors: Frampton, John P, White, Joshua B, Simon, Arlyne B, Tsuei, Michael, Paczesny, Sophie, Takayama, Shuichi
    Sci Rep, 2014;4(0):4878.
    Species: Human
    Sample Types: Plasma
  15. Daily exposure to dust alters innate immunity.
    Authors: Sahlander K, Larsson K, Palmberg L
    PLoS ONE, 2012;7(2):e31646.
    Species: Human
    Sample Types: Serum
  16. Soluble ST2 Associates with Diabetes but Not Established Cardiovascular Risk Factors: A New Inflammatory Pathway of Relevance to Diabetes?
    Authors: Miller A, Purves D, McConnachie A, Asquith D, Batty G, Burns H, Cavanagh J, Ford I, McLean J, Packard C, Shiels P, Turner H, Velupillai Y, Deans K, Welsh P, McInnes I, Sattar N
    PLoS ONE, 2012;7(10):e47830.
    Species: Human
    Sample Types: Plasma
  17. Soluble IL-1RII and IL-18 are associated with incipient upper extremity soft tissue disorders.
    Authors: Rechardt M, Shiri R, Matikainen S, Viikari-Juntura E, Karppinen J, Alenius H
    Cytokine, 2011;54(2):149-53.
    Species: Human
    Sample Types: Serum
  18. Primary sources and immunological prerequisites for sST2 secretion in humans.
    Authors: Mildner M, Storka A, Lichtenauer M
    Cardiovasc. Res., 2010;87(4):769-77.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Serum soluble ST2: a potential novel mediator in left ventricular and infarct remodeling after acute myocardial infarction.
    Authors: Weir RA, Miller AM, Murphy GE, Clements S, Steedman T, Connell JM, McInnes IB, Dargie HJ, McMurray JJ
    J. Am. Coll. Cardiol., 2010;55(3):243-50.
    Species: Human
    Sample Types: Serum
  20. Potential role of soluble ST2 protein in idiopathic nephrotic syndrome recurrence following kidney transplantation.
    Authors: Bruneau S, Le Berre L, Herve C, Valanciute A, Kamal M, Naulet J, Tesson L, Foucher Y, Soulillou JP, Sahali D, Dantal J
    Am. J. Kidney Dis., 2009;54(3):522-32.
    Species: Human
    Sample Types: Serum
  21. Induction of IRAK-M is associated with lipopolysaccharide tolerance in a human endotoxemia model.
    Authors: van 't Veer C, van den Pangaart PS, van Zoelen MA, de Kruif M, Birjmohun RS, Stroes ES, de Vos AF, van der Poll T
    J. Immunol., 2007;179(10):7110-20.
    Species: Human
    Sample Types: Plasma
  22. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.
    Species: Human
    Sample Types: Serum


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Reviews for Human ST2/IL-33R DuoSet ELISA

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Human ST2/IL-33R DuoSet ELISA
By Anonymous on 02/07/2020
Application: Sample Tested: EDTA Plasma

Normal human plasma range upto 500pg/ml used neat; plasma from cancer patients gives much much higher values (consider diluting plasma sample)

Human ST2/IL-33 R DuoSet ELISA
By Anonymous on 08/09/2017
Application: Sample Tested: EDTA Plasma

We ran this ELISA with human plasma samples at a 1:20 dilution reaching high precision.

Human ST2/IL-33 R DuoSet ELISA
By Anonymous on 05/08/2017
Application: Sample Tested: HUMAN SERUM