Human Stanniocalcin 1/STC-1 Antibody

Catalog # Availability Size / Price Qty
MAB2958
MAB2958-SP
Detection of Human Stanniocalcin 1/STC‑1 by Western Blot.
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Product Details
Citations (1)
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Human Stanniocalcin 1/STC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Stanniocalcin 1/STC‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Stanniocalcin 2 or recombinant mouse Stanniocalcin 1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 380715
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Stanniocalcin 1/STC‑1
Thr18-Ala247
Accession # P52823
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Stanniocalcin 1/STC‑1 by Western Blot. View Larger

Detection of Human Stanniocalcin 1/STC‑1 by Western Blot. Western blot shows lysates of human heart tissue, human kidney tissue, HT1080 human fibrosarcoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stanniocalcin 1/STC‑1 Monoclonal Antibody (Catalog # MAB2958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Stanniocalcin 1/STC‑1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Stanniocalcin 1/STC-1

Stanniocalcin-1 (STC-1) is a secreted 35 kDa disulfide-linked homodimer that is related to a calcium-regulating hormone in fish. Mature human Stanniocalcin-1 is 230 aa in length, and contains five intrachain disulfide bonds plus one interchain disulfide bond. Circulating Stanniocalcin-1 is both glycosylated and phosphorylated. Mature human Stanniocalcin-1 is 98%, 97%, 95% and 97% aa identical to mature canine, mouse, bovine and rat Stanniocalcin-1, respectively. Stanniocalcin-1 promotes phosphorus absorption and blocks chemokine-induced chemotaxis.

Entrez Gene IDs
6781 (Human)
Alternate Names
Stanniocalcin 1; stanniocalcin-1; STC1; STC-1; STCSTC-1

Product Datasheets

Citation for Human Stanniocalcin 1/STC-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells.
    Authors: He LF, Wang TT, Gao QY, Zhao GF, Huang YH, Yu LK, Hou YY
    J. Biomed. Sci., 2011;18(0):39.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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