Human Stanniocalcin 1/STC‑1 Antibody

Detection of Human Stanniocalcin 1/STC‑1 by Western Blot.

Western blot shows lysates of human heart tissue, human kidney tissue, HT1080 human fibrosarcoma cell line, and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Stanniocalcin 1/STC-1 Monoclonal Antibody (Catalog # MAB2958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Stanniocalcin 1/STC-1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Stanniocalcin 1/STC-1 by Immunocytochemistry/Immunofluorescence

Tumorigenesis and angiogenesis of BGC cells in nude mice. (A) Western blotting analysis of the expression of STC-1 in BGC after stable transfection. (B) Mean volumes of the tumor in each group were calculated. Cultured BGC cells and BGC stable transfection cells (106 cells) were injected subcutaneously into the flank of female nude mice. Tumor volumes were measured and calculated once every two days after we can see the tumor in the flank of nude mice. (C) Immunohistochemical staining of STC-1 in tumor tissues of nude mice. STC-1 was detected on the membrane of tumor cells (D) Immunohistochemical staining of PCNA in tumor tissues of nude mice. PCNA was detected in the nucleus of tumor cells. (E) Quantification of PCNA expression(the Integrated Optical Density (IOD) of PCNA) by image pro-plus software. All histology was carried out on multiple sections from individual mice and three independent in vivo experiments. (F) Hematoxylin and eosin stained sections of the Matrigel plugs (E, endothelial-like cells; T, tumor cells; S, surrounding tissues; V, microvessels). (G) Mean vessel area was quantified in each group. (*P < 0.05, **P < 0.01). Image collected and cropped by CiteAb from the following publication (https://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-18-39), licensed under a CC-BY license. Not internally tested by R&D Systems.