Human STAT2 Antibody

R&D Systems | Catalog # PAF-ST2

R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all STAT2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STAT2
Gln679-Phe851
Accession # P52630

Specificity

Detects human STAT2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human STAT2 Antibody

Western Blot Shows Human STAT2 Specificity by Using Knockout Cell Line.

Western Blot Shows Human STAT2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT2 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human STAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST2) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Shows Human STAT2 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human STAT2 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line, STAT1 knockout (KO) HeLa cell line, STAT2 KO HeLa cell line, STAT3 KO HeLa cell line, STAT5a KO HeLa cell line, STAT5b KO HeLa cell line, and STAT6 KO HeLa cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human STAT2 Antigen Affinity-purified Polyclonal Antibody (Catalog # PAF-ST2) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

IFN-beta and IFN-lambda mediate STAT1 and STAT2 activation upon RSV and IAV infection in A549 cells. (A and B) ELISA for IFN-beta following RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Open circles mark data points with cytokine concentrations below the detection range. Error bars are standard deviations (SD) from technical replicates. (C) IFN-beta (green) (left) and activation of STAT1 (yellow) (right) and IRF3 (red) 24 h after infection of A549 WT cells with RSV (magenta, polyclonal anti-RSV antibody) at an MOI of 0.01. White dotted lines are nuclear outlines determined based on DAPI counterstaining (channel not shown). Bars, 50 μm. (D and E) Activation of STAT1, STAT2, and interferon-stimulated genes (RIG-I, PKR, and OAS1) upon RSV (D) and IAV infection (E) at an MOI of 0.1 at 24 and 48 h postinfection (p.i.) in A549 WT, IFNAR1 KO, IFNLR1 KO, and IFNAR1-IFNLR1 dKO cells. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. RSV F, RSV fusion glycoprotein; IAV NP, IAV nucleoprotein. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of STAT2 by Western Blot

Detection of STAT2 by Western Blot

Prestimulation with IFN-beta or IFN-lambda 1 inhibits the propagation of RSV and IAV in A549 WT cells. (A and B) Effect of prestimulation with IFN-beta (1,000 U/mL) or IFN-lambda 1 (50 ng/mL) 24 h prior to infection on the relative abundance of viral RNA 24 h after RSV (A) and IAV (B) infection at MOIs of 0.1 and 1. Each filled circle corresponds to fold changes compared to nonprestimulated cells in a single experimental replicate (note the logarithmic scale). Error bars show the smallest and the largest ratios of nonprestimulated to IFN-prestimulated cells for all pairs of the respective technical replicates. (C and D) Effect of prestimulation with IFN-beta (1,000 U/mL) 24 h prior to infection on the abundance and activation of STAT1 and STAT2 and the abundance of interferon-stimulated genes (RIG-I, PKR, and OAS1) and the RSV fusion glycoprotein (RSV F) (C) and IAV nucleoprotein (IAV NP) (D) at 24 and 48 h postinfection (p.i.) at MOIs of 0.1 and 1. n.t. indicates nontreated cells. Activated STATs p-STAT1 and p-STAT2 are STAT1 and STAT2 phosphorylated at Tyr701 and Tyr690, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36326278), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human STAT2 Antibody

Application
Recommended Usage

Immunoprecipitation

3 µg/106 cells
Sample: WS‑1 human fetal skin fibroblast cell line, see our available Western blot detection antibodies

Knockout Validated

STAT2 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT2 knockout HeLa cell line.

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 1 mg/mL in deionized water.

Formulation

Lyophilized from a 0.2 μm sterile-filtered solution in phosphate-buffered saline (PBS) with 5% trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: STAT2

STAT2 (Signal Transducer and Activator of Transcription #2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with Type I ( alpha - and beta -) Interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y689 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name

Signal Transducer and Activator of Transcription 2

Alternate Names

interferon alpha induced transcriptional activator, ISGF-3, MGC59816, P113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113

Entrez Gene IDs

6773 (Human); 20847 (Mouse)

Gene Symbol

STAT2

UniProt

P52630

Additional STAT2 Products

Product Documents for Human STAT2 Antibody

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Product Specific Notices for Human STAT2 Antibody

For research use only

Citations for Human STAT2 Antibody

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