Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1277A
View all Survivin Primary Antibodies »

Product Specifications

Immunogen

Full length recombinant human Survivin
Accession # O15392

Specificity

Detects human Survivin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Survivin Antibody

Detection of Survivin in Human Breast CancerTissue via seqIF™ staining on COMET™

Survivin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Survivin Monoclonal Antibody (Catalog # MAB8861) at 15ug at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell nucleus. Protocol available in COMET™ Panel Builder.
Detection of Human Survivin antibody by Western Blot.

Detection of Human Survivin by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 786-O human renal cell adenocarcinoma cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Survivin Polyclonal Antibody (Catalog # MAB8861) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Survivin at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Survivin antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

Survivin in MCF‑7 Human Cell Line.

Survivin was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human Survivin Polyclonal Antibody (Catalog # MAB8861) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Survivin antibody by Simple WesternTM.

Detection of Human Survivin by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Survivin at approximately 23 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Survivin Monoclonal Antibody (Catalog # MAB8861). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

Survivin in Human Breast Cancer Tissue

Survivin was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human Survivin Monoclonal Antibody (Catalog # MAB8861) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to and cytoplasm and nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Survivin Antibody

Application
Recommended Usage

Immunocytochemistry

1 µg/mL
Sample: MCF‑7 human breast cancer cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue

Multiplex Immunofluorescence

15 µg/mL
Sample: Immersion Fixed Paraffin-Embedded sections of human breast cancer. 

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, 786‑O human renal cell adenocarcinoma cell line, and Bowes human melanoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB8861 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Survivin

Survivin also known as Apoptosis inhibitor survivin and Baculoviral IAP repeat-containing protein 5 (BIRC5) is an 18 kDa member of the inhibitor of apoptosis (IAP) gene family, which encodes negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple Baculovirus IAP repeat (BIR) domains, but this gene encodes seven isoforms with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Survivin is expressed in a cell cycle-dependent manner and associates with the mitotic apparatus.

Alternate Names

API4, BIRC5

Entrez Gene IDs

332 (Human)

Gene Symbol

BIRC5

UniProt

O15392

Additional Survivin Products

Product Documents for Human Survivin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Survivin Antibody

For research use only

Citations for Human Survivin Antibody

Customer Reviews for Human Survivin Antibody (1)

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  • Human Survivin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Breast cancer tissue
    Species: Human
    Verified Customer | Posted 02/08/2022
    Human Survivin Antibody MAB8861

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Protocols

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FAQs

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail