Detection of SUSD2 by Western Blot.
Western blot shows lysates of SK‑BR‑3 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for SUSD2 at approximately 110 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.
Sushi Domain Containing 2 (SUSD2) was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective Isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibits colon cancer cell growth through inducing G1 arrest. There is evidence that SUSD2 may play a role in breast tumorigenesis.
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