Human SUSD2 Antibody

Recombinant Monoclonal Antibody..
  
  • Species Reactivity
    Human
  • Specificity
    Detects human SUSD2 in direct ELISAs and Western blots.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1279A
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line-derived human SUSD2
    Met1-Ala785
    Accession # Q9UGT4
  • Formulation
    Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunohistochemistry
    3-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SUSD2 by Western Blot. Western blot shows lysates of SK‑BR‑3 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for SUSD2 at approximately 110 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of SUSD2 in HEK293 Human Cell Line Transfected with Human SUSD2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human SUSD2 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.
Immunohistochemistry
SUSD2 in Human Breast Cancer Tissue.

Sushi Domain Containing 2 (SUSD2) was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Rabbit Anti-Human SUSD2 Monoclonal Antibody (Catalog # MAB90562) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: SUSD2
Sushi domain containing 2, or SUSD2, is a type I transmembrane protein of 822 amino acids containing functional domains inherent to adhesion molecules. SUSD2 has been described as a novel marker of human endometrial mesenchymal stem-like cells and it has been used for their prospective Isolation. As a transmembrane receptor, SUSD2 has been proposed to interact with Galectin-1 and to be the receptor for C10ORF99, a novel potential cytokine suggested to inhibits colon cancer cell growth through inducing G1 arrest. There is  evidence that SUSD2 may play a role in breast tumorigenesis.
  • Long Name:
    Sushi Domain Containing 2
  • Entrez Gene IDs:
    56241 (Human); 71733 (Mouse); 294335 (Rat)
  • Alternate Names:
    BK65A6.2; FLJ22778; SUSD2; Sushi domain (SCR repeat) containing; sushi domain containing 2; sushi domain-containing protein 2
Related Research Areas
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Basic

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Antigen Retrieval Reagent Sampler (50 mL each)

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Mounting Medium

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NorthernLights Guard Mounting Media

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Isotype Controls
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Normal Rabbit IgG Control

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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

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Secondary Antibodies
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Rabbit IgG Horseradish Peroxidase-conjugated Antibody

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Rabbit IgG PE-conjugated Antibody

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Donkey Anti-Rabbit IgG NL557 Affinit Purified PAb

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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

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Rabbit IgG APC-conjugated Antibody

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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 2
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 2  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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