Human Synaptopodin Antibody

Catalog # Availability Size / Price Qty
MAB8977
MAB8977-SP
Synaptopodin  in Human Kidney.
1 Image
Product Details
Citations (1)
FAQs
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Human Synaptopodin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Synaptopodin in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 918842
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Synaptopodin
Ser363-Gln490
Accession # Q8N3V7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Synaptopodin  in Human Kidney. View Larger

Synaptopodin in Human Kidney. Synaptopodin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Synaptopodin Monoclonal Antibody (Catalog # MAB8977) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Synaptopodin

Synaptopodin is a 929 amino acid, actin associated protein with restricted expression in brain and kidney.  First reported in glomerular podocytes, the protein is also  found in postsynaptic densities (PSDs) in a subpopulation of telencephalic neurons where it is thought to participate in synaptic plasticity in dendritic spines.  Despite its localization in PSDs, it is not homologous to other PSD proteins.  Loss of synaptopodin expression has been demonstrated in Alzheimer’s patients. In glomeruli, synaptopodin localizes to the cytoplasm of podocyte foot processes. 

Entrez Gene IDs
11346 (Human); 104027 (Mouse); 60324 (Rat)
Alternate Names
KIAA1029; Synaptopodin; SYNPO

Product Datasheets

Citation for Human Synaptopodin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Blockage of the lysosome-dependent autophagic pathway contributes to complement membrane attack complex-induced podocyte injury in idiopathic membranous nephropathy
    Authors: WJ Liu, ZH Li, XC Chen, XL Zhao, Z Zhong, C Yang, HL Wu, N An, WY Li, HF Liu
    Sci Rep, 2017;7(1):8643.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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