Synaptopodin in Human Kidney. Synaptopodin was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Synaptopodin Monoclonal Antibody (Catalog # MAB8977) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Synaptopodin is a 929 amino acid, actin associated protein with restricted expression in brain and kidney. First reported in glomerular podocytes, the protein is also found in postsynaptic densities (PSDs) in a subpopulation of telencephalic neurons where it is thought to participate in synaptic plasticity in dendritic spines. Despite its localization in PSDs, it is not homologous to other PSD proteins. Loss of synaptopodin expression has been demonstrated in Alzheimer’s patients. In glomeruli, synaptopodin localizes to the cytoplasm of podocyte foot processes.
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