Human Syndecan-1/CD138 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4,and recombinant mouse Syndecan-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
    Gln18-Glu251
    Accession # NP_002988
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Human Syndecan-1/CD138 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human Syndecan‑1/CD138 Antibody (Catalog # AF2780)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Syndecan‑1/CD138 Biotinylated Antibody (Catalog # BAF2780)
  • ELISA Standard
     
    Recombinant Human Syndecan-1 Protein, CF (Catalog # 2780-SD)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Syndecan‑1/CD138 in Human Intestine. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human intestine using Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syndecan-1/CD138

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

  • References:
    1. Entrez Gene IDs:
      6382 (Human); 20969 (Mouse); 100338470 (Rabbit)
    2. Alternate Names:
      CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citations: Showing 1 - 1

    1. Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
      Authors: Cheshenko N, Liu W, Satlin LM, Herold BC
      Mol. Biol. Cell, 2007;18(8):3119-30.
      Species: Human
      Sample Type: Cell Lysates
      Application: WB
    Immunohistochemistry Reagents
    Description Application Cat# Citations Images  

    Antigen Retrieval Reagent-Universal

    CTS015 10
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    Antigen Retrieval Reagent-Basic

    CTS013 4
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    Mounting Medium

    CTS011 4
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    Antigen Retrieval Reagent Sampler (50 mL each)

    CTS016
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    Antigen Retrieval Reagent-Acidic

    CTS014
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    NorthernLights Guard Mounting Media

    NL996
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    VisUCyte Antigen Retrieval Reagent-Acidic

    VCTS022
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    VisUCyte Antigen Retrieval Reagent-Universal

    VCTS023
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    VisUCyte Antigen Retrieval Reagent-Basic

    VCTS021
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    Isotype Controls
    Description Application Cat# Citations Images  

    Normal Goat IgG Biotinylated Control

    Ctrl BAF108 19
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    Staining Reagents
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    Streptavidin-Phycoerythrin

    Flow F0040 7
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    Streptavidin-Alkaline Phosphatase

    ELISA(Det) AR001 2
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    NorthernLights Streptavidin NL557

    Flow, IHC, ICC NL999 4
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    NorthernLights Streptavidin NL637

    Flow, IHC, ICC NL998 2
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    NorthernLights Streptavidin NL493

    Flow, IHC, ICC NL997 1
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    Streptavidin-Allophycocyanin

    Flow F0050 1
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    Avidin-Fluorescein

    Flow F0030 1
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    Supplemental Cell Selection Products
    Description Application Cat# Citations Images  

    MagCellect Streptavidin Ferrofluid

    MAG999B 4
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    MagCellect Streptavidin Ferrofluid

    MAG999 4
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Streptavidin-HRP

    ELISA DY998 44
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    EvenCoat Streptavidin Coated Plates

    ELISA CP004
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    EvenCoat Streptavidin Coated Plates, 5 Pack

    ELISA CP003 3
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