Human Syndecan-1/CD138 Biotinylated Antibody

Catalog # Availability Size / Price Qty
Syndecan‑1/CD138 in Human Intestine.
2 Images
Product Details
Citations (1)
Supplemental Products

Human Syndecan-1/CD138 Biotinylated Antibody Summary

Species Reactivity
Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4,and recombinant mouse Syndecan-1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Accession # NP_002988
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD)
3-15 µg/mL
Immersion fixed paraffin-embedded sections of Human Liver

Human Syndecan-1/CD138 Sandwich Immunoassay

Recommended Concentration
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human Syndecan‑1/CD138 Antibody (Catalog # AF2780)

Standard: Recombinant Human Syndecan-1 Protein, CF (Catalog # 2780-SD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Syndecan‑1/CD138 antibody in Human Intestine by Immunohistochemistry (IHC-P). View Larger

Syndecan‑1/CD138 in Human Intestine. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human intestine using Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry View Larger

Detection of Syndecan‑1/CD138 in Human Liver. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of Human Liver using Goat Anti-Human Syndecan‑1/CD138 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2780) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-1/CD138

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

Entrez Gene IDs
6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)
Alternate Names
CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1

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Citation for Human Syndecan-1/CD138 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Multiple receptor interactions trigger release of membrane and intracellular calcium stores critical for herpes simplex virus entry.
    Authors: Cheshenko N, Liu W, Satlin LM, Herold BC
    Mol. Biol. Cell, 2007;18(8):3119-30.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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