Human Syndecan-1/CD138 Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
    Gln18-Glu251
    Accession # NP_002988
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD)
  • Immunohistochemistry
    5-15 µg/mL
    See below
    • Human Syndecan-1/CD138 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human Syndecan‑1/CD138 Antibody (Catalog # AF2780)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Syndecan‑1/CD138 Biotinylated Antibody (Catalog # BAF2780)
  • ELISA Standard
     
    Recombinant Human Syndecan-1 Protein, CF (Catalog # 2780-SD)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Syndecan‑1/CD138 in Human Ileum. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
Syndecan‑1/CD138 in Human Jejunum. Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Syndecan-1/CD138

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

  • References:
    1. Entrez Gene IDs:
      6382 (Human); 20969 (Mouse); 100338470 (Rabbit)
    2. Alternate Names:
      CD138 antigen; CD138; SDC; SDC1; SYND1heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; syndecan proteoglycan 1; syndecan; Syndecan1; Syndecan-1
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    6 Citations: Showing 1 - 6
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    Species
    Applications
    Sample Type
    1. Isolation of Circulating Plasma Cells in Multiple Myeloma Using CD138 Antibody-Based Capture in a Microfluidic Device
      Authors: MA Qasaimeh, YC Wu, S Bose, A Menachery, S Talluri, G Gonzalez, M Fulciniti, JM Karp, RH Prabhala, R Karnik
      Sci Rep, 2017;7(0):45681.
      Species: Human
      Sample Type: Whole Cells
      Application: IP
    2. Fibronectin on the Surface of Myeloma Cell-derived Exosomes Mediates Exosome-Cell Interactions.
      Authors: Purushothaman A, Bandari S, Liu J, Mobley J, Brown E, Sanderson R
      J Biol Chem, 2016;291(4):1652-63.
      Species: Human
      Sample Type: Whole Cells
      Application: Flow
    3. Shed syndecan-1 translocates to the nucleus of cells delivering growth factors and inhibiting histone acetylation: a novel mechanism of tumor-host cross-talk.
      Authors: Stewart M, Ramani V, Sanderson R
      J Biol Chem, 2015;290(2):941-9.
      Species: Human
      Sample Type: Cell Lysates
      Application: WB
    4. Novel processed form of syndecan-1 shed from SCC-9 cells plays a role in cell migration.
      Authors: Aragao A, Belloni M, Simabuco F, Zanetti M, Yokoo S, Domingues R, Kawahara R, Pauletti B, Goncalves A, Agostini M, Graner E, Coletta R, Fox J, Leme A
      PLoS ONE, 2012;7(8):e43521.
      Species: Human
      Sample Type: Whole Cells
      Application: ICC
    5. Heparanase Plays a Dual Role in Driving Hepatocyte Growth Factor (HGF) Signaling by Enhancing HGF Expression and Activity.
      Authors: Ramani VC, Yang Y, Ren Y
      J. Biol. Chem., 2011;286(8):6490-9.
      Species: Human
      Sample Type: Cell Culture Supernates
      Application: Immunodepletion
    6. CD28-mediated regulation of multiple myeloma cell proliferation and survival.
      Authors: Bahlis NJ, King AM, Kolonias D, Carlson LM, Liu HY, Hussein MA, Terebelo HR, Byrne GE, Levine BL, Boise LH, Lee KP
      Blood, 2007;109(11):5002-10.
      Species: Human
      Sample Type: Whole Tissue
      Application: IHC
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