Human Syndecan-1/CD138 Antibody

R&D Systems | Catalog # AF2780

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Microarray, Immunodepletion

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Syndecan-1/CD138 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-1/CD138
Gln18-Glu251
Accession # NP_002988

Specificity

Detects human Syndecan-1/CD138 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human (rh) Syndecan-2, rhSyndecan-3, rhSyndecan-4, and recombinant mouse Syndecan-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Syndecan-1/CD138 Antibody

Syndecan‑1/CD138 antibody in Human Ileum by Immunohistochemistry (IHC-P).

Syndecan‑1/CD138 in Human Ileum.

Syndecan‑1/CD138 was detected in immersion fixed paraffin-embedded sections of human ileum using Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Syndecan-1/CD138 antibody in Human Jejunum by Immunohistochemistry (IHC-P).

Syndecan‑1/CD138 in Human Jejunum.

Syndecan-1/CD138 was detected in immersion fixed paraffin-embedded sections of human jejunum using Human Syndecan-1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human Syndecan-1 / CD138 ELISA Standard Curve

Human Syndecan-1 / CD138 ELISA Standard Curve

Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD) was serially diluted and captured by Goat Anti-Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Human Syndecan-1 / CD138 ELISA Standard Curve

Human Syndecan-1 / CD138 ELISA Standard Curve

Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD) was serially diluted and captured by Goat Anti-Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Syndecan‑1/CD138 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2780) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of Syndecan-1 by Immunohistochemistry

Detection of Syndecan-1 by Immunohistochemistry

Immunostaining of CX3CL1 and CX3CR1 in SSc-ILD.CX3CL1 protein is produced from the epithelium and infiltrating interstitial leukocytes in SSc-ILD, while its receptor CX3CR1 is localized to infiltrating mononuclear cells. Representative histopathological staining of (n = 5) SSc-ILD for CX3CL1 from (A) Type 2 pneumocytes, (B) Airway epithelium, (C) Epithelium involved in bronchiolization, and (D) Infiltrating mononuclear cells. Representative histopathological staining of (n = 5) SSc-ILD for CX3CR1 from (E) Infiltrating interstitial mononuclear cells, (F) Morphologically from plasma cells via their eccentric cartwheel nuclei, (G) Representative staining of plasma cell marker CD138 confirming that the cells with eccentric cartwheel nuclei are plasma cells, (H) Representative immunofluorescence staining of plasma cell (red) in the interstitium, CX3CR1 positive infiltrating mononuclear cells, and co-localization of CD138+ plasma cell expressing CX3CR1 and infiltrating the interstitium. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30457999), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Syndecan-1 by Immunohistochemistry

Detection of Syndecan-1 by Immunohistochemistry

Immunostaining of CX3CL1 and CX3CR1 in SSc-ILD.CX3CL1 protein is produced from the epithelium and infiltrating interstitial leukocytes in SSc-ILD, while its receptor CX3CR1 is localized to infiltrating mononuclear cells. Representative histopathological staining of (n = 5) SSc-ILD for CX3CL1 from (A) Type 2 pneumocytes, (B) Airway epithelium, (C) Epithelium involved in bronchiolization, and (D) Infiltrating mononuclear cells. Representative histopathological staining of (n = 5) SSc-ILD for CX3CR1 from (E) Infiltrating interstitial mononuclear cells, (F) Morphologically from plasma cells via their eccentric cartwheel nuclei, (G) Representative staining of plasma cell marker CD138 confirming that the cells with eccentric cartwheel nuclei are plasma cells, (H) Representative immunofluorescence staining of plasma cell (red) in the interstitium, CX3CR1 positive infiltrating mononuclear cells, and co-localization of CD138+ plasma cell expressing CX3CR1 and infiltrating the interstitium. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30457999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Syndecan-1/CD138 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine and immersion fixed paraffin-embedded sections of normal human ileum and jejunum

Western Blot

0.1 µg/mL
Sample: Recombinant Human Syndecan‑1/CD138 (Catalog # 2780-SD)

Human Syndecan-1/CD138 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Syndecan‑1/CD138 Biotinylated Antibody (Catalog # BAF2780)
  • Standard: Recombinant Human Syndecan-1 Protein, CF (Catalog # 2780-SD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF2780 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Syndecan-1/CD138

Human Syndecan-1 (also known as CD138) is a variably glycosylated, dimeric, type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 310 amino acid (aa) precursor that contains a 17 aa signal sequence, a 234 aa extracellular domain (ECD), a 25 aa TM segment, and a 34 aa cytoplasmic region. The ECD shows various degrees of heparan sulfate and chondroitin sulfate modification, leading to native molecular weights for Syndecan-1 of 120 - 200 kDa. Proteolytic cleavage of the membrane-bound ECD yields soluble forms of approximately the same molecular weight. Syndecan-1 is an epithelial cell Syndecan involved in Wnt and chemokine signaling. Human Syndecan-1 ECD shares 71% aa identity with the ECD of both rat and mouse Syndecan-1.

References

    Alternate Names

    CD138, SDC1, Syndecan1

    Entrez Gene IDs

    6382 (Human); 20969 (Mouse); 100519770 (Porcine); 100338470 (Rabbit)

    Gene Symbol

    SDC1

    UniProt

    NP_002988

    Additional Syndecan-1/CD138 Products

    Product Documents for Human Syndecan-1/CD138 Antibody

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

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    Product Specific Notices for Human Syndecan-1/CD138 Antibody

    For research use only

    Citations for Human Syndecan-1/CD138 Antibody

    Customer Reviews for Human Syndecan-1/CD138 Antibody (1)

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    • Name: Natalie Erdmann
      Application: Immunofluorescence
      Sample Tested: RPMI-8226 multiple myeloma cell line, and Daudi Burkitt lymphoma cell line
      Species: Human
      Verified Customer | Posted 03/13/2015
      AF2780 immunofluorescence staining in cell lines
      Human Syndecan-1/CD138 Antibody AF2780

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