Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TBX3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TBX3
Ser321-Thr483
Accession # O15119

Specificity

Detects human TBX3 in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TBX2, rhTBX6, and rhTBX20 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TBX3 Antibody

Detection of Human TBX3 antibody by Western Blot.

Detection of Human TBX3 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for TBX3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Detection of Human TBX3 by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TBX3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

TBX3 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

TBX3 in LNCaP Human Cell Line.

TBX3 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human TBX3 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed ZR-75-1 human mammary ductal carcinoma cell line and LNCaP human prostate cancer cell line

Western Blot

1-2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using AF4509 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TBX3

TBX3 belongs to the T-box family of transcription factors, which are involved in regulation of developmental processes. TBX3 acts as a transcriptional repressor regulating anterior/posterior axis of the forelimb. Mutations in TBX3 are associated with ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. TBX3 has 3 splice isoforms.

Long Name

T-box Transcription Factor 3

Alternate Names

UMS, XHL

Entrez Gene IDs

6926 (Human); 21386 (Mouse); 353305 (Rat)

Gene Symbol

TBX3

UniProt

O15119

Additional TBX3 Products

Product Documents for Human TBX3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TBX3 Antibody

For research use only

Citations for Human TBX3 Antibody

Customer Reviews for Human TBX3 Antibody (1)

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1 Customer Rating
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Customer Images

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: R1 wild type Mouse embryonic Stem Cells
    Species: Mouse
    Verified Customer | Posted 08/04/2016
    Tbx3 in R1 wild type mouse embryonic stem cells
    Human TBX3 Antibody AF4509

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Protocols

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