Detection of Human TCF-12/HTF4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TCF-12/HTF4 Monoclonal Antibody (Catalog # MAB6928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF-12/HTF4 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TCF-12 (transcription factor 12), also called HTF4, bHLHb20 and HEB, is a 682 amino acid (aa) DNA binding protein of the E protein family of helix-loop-helix (HLH) transcription factors. E proteins, including TCF-12 and E2A, mediate lymphocyte differentiation and also function in neurogenesis. Homo-oligomerization is required for activity; hetero-oligomers are also formed. The region of human TCF-12 used as an immunogen excludes the HLH domain and shares 94% aa sequence identity with mouse and rat TCF-12. Potential isoforms of 706 and 648 aa contain an insertion of 24 aa after aa 396, while isoforms of 648, 624 and 512 aa show alternate N‑terminal sequences that merge with the 682 aa form at aa 75, 75 and 194, respectively.
Transcription Factor 12
Entrez Gene IDs:
6938 (Human); 21406 (Mouse); 25720 (Rat)
BHLHB20; bHLHb20E-box-binding protein; Class B basic helix-loop-helix protein 20; HEB; HEBDNA-binding protein HTF4; helix-loop-helix transcription factor 4; HsT17266; HTF4; HTF4TCF-12; TCF12; transcription factor 12; Transcription factor HTF-4
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