Human TCF7L1/TCF3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human TCF7L1/TCF3 in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 622722
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human TCF7L1/TCF3
    Lys429-Ser581
    Accession # Q9HCS4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TCF7L1 by Western Blot.

Western blot shows lysates of human lung tissue, human pancreas tissue, and human spleen tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human TCF7L1/TCF3 Monoclonal Antibody (Catalog # MAB6116) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TCF7L1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution

    Reconstitute at 0.5 mg/mL in sterile PBS.

  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TCF7L1/TCF3
Transcription factor 7 like 1 (TCF7L1; also HMG box transcription factor 3, TCF3) is a 63 kDa member of the TCF/LEF family of proteins. Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346414). Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1. TCF7L1 is expressed in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. Functionally, it participates in the Wnt signaling pathway. TCF7L1 binds to DNA and acts as a repressor in the absence of beta-Catenin, and as an activator in its presence. TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.
  • Long Name:
    Transcription Factor 7-like 1
  • Entrez Gene IDs:
    83439 (Human); 21415 (Mouse); 312451 (Rat)
  • Alternate Names:
    HMG box transcription factor 3; TCF3; TCF-3; TCF7L1; transcription factor 7-like 1 (T-cell specific, HMG-box); transcription factor 7-like 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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