Human TfR2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human TfR2 by Western Blot.
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Product Details
Supplemental Products

Human TfR2 Antibody Summary

Species Reactivity
Detects human TfR2 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 353810
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human TfR2
Accession # Q9UP52
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
K562 human chronic myelogenous leukemia cell line
Flow Cytometry
0.25 µg/106 cells
 K562 human myelogenous leukemia cell line
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TfR2 antibody by Western Blot. View Larger

Detection of Human TfR2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB31202) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TfR2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of TfR2 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TfR2 in K562 Human Cell Line by Flow Cytometry. K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human TfR2 Monoclonal Antibody (Catalog # MAB31202, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TfR2

Transferrin Receptor 2 (TfR2) is an iron-binding protein that is homologous to the transferrin receptor. Lack of active TfR2 has been associated with a rare form of hemochromatosis. The TfR2 alpha isoform is a type 2 transmembrane protein that is expressed in the liver and modulates hepcidin production in response to iron. It forms a 215 kDa disulfide-linked homodimer that is presumably glycated. The TfR2 beta isoform lacks the intracellular, transmembrane and part of the extracellular regions and is expressed ubiquitously. The extracellular portion of human TfR2 shares 86% aa identity with mouse.

Long Name
Transferrin Receptor 2
Entrez Gene IDs
7036 (Human); 50765 (Mouse)
Alternate Names
HFE3; HFE3transferrin receptor protein 2; MGC126368; TfR2; TFRC2; transferrin receptor 2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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