Human Thrombospondin-4 Antibody

Catalog # Availability Size / Price Qty
Detection of Human Thrombospondin‑4 by Western Blot.
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Product Details
Citations (4)
Supplemental Products
Reviews (3)

Human Thrombospondin-4 Antibody Summary

Species Reactivity
Detects human Thrombospondin-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human Thrombospondin-1, -2, or -3.
Monoclonal Mouse IgG2B Clone # 276523
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Thrombospondin-4 antibody by Western Blot. View Larger

Detection of Human Thrombospondin‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thrombospondin-4 Monoclonal Antibody (Catalog # MAB2390) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Thrombospondin-4 (TSP-4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. TSP‑1 and -2 constitute subgroup A and form disulfide-linked homotrimers, whereas TSP-3, -4, and -5/COMP constitute subgroup B and form pentamers (1, 2). The human TSP-4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven TSP type-3 repeats (one with an RGD motif), and a TSP C-terminal domain (3). Human TSP-4 shares 93% aa sequence identity with mouse and rat TSP-4. Within the TSP type-3 repeats and the TSP C-terminal domain, human TSP-4 shares 79% aa sequence identity with TSP-3 and COMP, and 58% aa sequence identity with TSP-1 and -2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). TSP-4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of TSP-4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). TSP-4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). TSP-4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of TSP-4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type TSP-4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type TSP-4, although the A387P variant induces a greater release of proinflammatory molecules (13).

  1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
  3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
  6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
  7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
  8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  9. Stenina, O.I. et al. (2003) Circulation 108:1514.
  10. Topol, E.J. et al. (2001) Circulation 104:2641.
  11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
  12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
  13. Pluskota, E. et al. (2005) Blood 106:3970.
Entrez Gene IDs
7060 (Human); 21828 (Mouse)
Alternate Names
THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

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Citations for Human Thrombospondin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The clinicopathological significance of Thrombospondin-4 expression in the tumor microenvironment of gastric cancer
    Authors: K Kuroda, M Yashiro, T Sera, Y Yamamoto, Y Kushitani, A Sugimoto, S Kushiyama, S Nishimura, S Togano, T Okuno, T Tamura, T Toyokawa, H Tanaka, K Muguruma, M Ohira
    PLoS ONE, 2019;14(11):e0224727.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Thrombospondin-4 reduces binding affinity of [(3)H]-gabapentin to calcium-channel ?2?-1-subunit but does not interact with ?2?-1 on the cell-surface when co-expressed
    Authors: B Lana, KM Page, I Kadurin, S Ho, M Nieto-Rost, AC Dolphin
    Sci Rep, 2016;6(0):24531.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.
    Authors: Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W
    Mod. Pathol., 2011;24(10):1390-403.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Thrombospondin-4 is a putative tumour-suppressor gene in colorectal cancer that exhibits age-related methylation.
    Authors: Greco SA, Chia J, Inglis KJ
    BMC Cancer, 2010;10(0):494.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P


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Reviews for Human Thrombospondin-4 Antibody

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Human Thrombospondin-4 Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Thrombospondin-4 Antibody
By Anonymous on 10/04/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody for a sandwich ELISA in combination with pAb (AF2390)) and protein (2390-TH). This combination works very well for detecting the TSP4 in human serum and plasma

Human Thrombospondin-4 Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human