Human Thrombospondin-4 Antibody

Catalog # Availability Size / Price Qty
AF2390
AF2390-SP
Human Thrombospondin-4 Antibody in Data
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (4)

Human Thrombospondin-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Thrombospondin-4 is observed and less than 5% cross-reactivity with recombinant human (rh) Thrombospondin-1, rhThrombospondin-2, and rhThrombospondin-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Thrombospondin‑4 by Western Blot. View Larger

Detection of Human Thrombospondin‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Thrombospondin‑4 by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human Thrombospondin‑4 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombospondin-4

Thrombospondin-4 (THSP4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. THSP1 and THSP2 constitute subgroup A and form disulfide-linked homotrimers, whereas THSP3, THSP4, and THSP5/COMP constitute subgroup B and form pentamers (1, 2). The human THSP4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven THSP type-3 repeats (one with an RGD motif), and a THSP C‑terminal domain (3). Human THSP4 shares 93% aa sequence identity with mouse and rat THSP4. Within the THSP type-3 repeats and the THSP C‑terminal domain, human THSP4 shares 79% aa sequence identity with THSP3 and COMP, and 58% aa sequence identity with THSP1 and THSP2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). THSP4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of THSP4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). THSP4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). THSP4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of THSP4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type THSP4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type THSP4, although the A387P variant induces a greater release of pro-inflammatory molecules (13).

References
  1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
  3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
  6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
  7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
  8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  9. Stenina, O.I. et al. (2003) Circulation 108:1514.
  10. Topol, E.J. et al. (2001) Circulation 104:2641.
  11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
  12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
  13. Pluskota, E. et al. (2005) Blood 106:3970.
Entrez Gene IDs
7060 (Human); 21828 (Mouse)
Alternate Names
THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4

Product Datasheets

Citations for Human Thrombospondin-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. Thrombospondin-4 mediates TGF-?-induced angiogenesis
    Authors: S Muppala, R Xiao, I Krukovets, D Verbovetsk, R Yendamuri, N Habib, P Raman, E Plow, O Stenina-Ad
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Assessing the contribution of thrombospondin-4 induction and ATF6? activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction
    Sci Rep, 2016;6(0):32449.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Thrombospondin expression in myofibers stabilizes muscle membranes
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Thrombospondin 4 deficiency in mouse impairs neuronal migration in the early postnatal and adult brain.
    Authors: Girard F, Eichenberger S, Celio M
    Mol Cell Neurosci, 2014;61(0):176-86.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013;497(7449):369-73.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.
    Authors: Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W
    Mod. Pathol., 2011;24(10):1390-403.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Increased cortical expression of two synaptogenic thrombospondins in human brain evolution.
    Authors: Caceres M, Suwyn C, Maddox M, Thomas JW, Preuss TM
    Cereb. Cortex, 2007;17(10):2312-21.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Thrombospondin-4 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

5 Star
75%
4 Star
0%
3 Star
25%
2 Star
0%
1 Star
0%

Have you used Human Thrombospondin-4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Thrombospondin-4 Antibody
By Anonymous on 03/14/2020
Application: IHC Sample Tested: Embryo Species: Zebrafish

The antibody was used to detect human TSP4 protein (Red channel in the figure) that was injected into a zebrafish embryo. The antibody did not cross-react with zebrafish Tsp4 protein.
The data has been published (eLife 2014;3:e02372).


Human Thrombospondin-4 Antibody
By Anonymous on 10/04/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

We used this antibody for a sandwich ELISA in combination with mAb (MAB2390)) and protein (2390-TH). This combination works very well for detecting the TSP4 in human serum and plasma


Human Thrombospondin-4 Antibody
By Anonymous on 11/08/2018
Application: Luminex Sample Tested: Serum Species: Human

Human Thrombospondin-4 Antibody
By Anonymous on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human