Human Thrombospondin-4 Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Thrombospondin-4 is observed and less than 5% cross-reactivity with recombinant human (rh) Thrombospondin-1, rhThrombospondin-2, and rhThrombospondin-3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
    Ala22-Asn961 (Pro276Ala, Ala420Val)
    Accession # P35443
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Thrombospondin‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thrombospondin‑4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Thrombospondin‑4 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin‑4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombospondin-4

Thrombospondin-4 (THSP4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. THSP1 and THSP2 constitute subgroup A and form disulfide-linked homotrimers, whereas THSP3, THSP4, and THSP5/COMP constitute subgroup B and form pentamers (1, 2). The human THSP4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven THSP type-3 repeats (one with an RGD motif), and a THSP C‑terminal domain (3). Human THSP4 shares 93% aa sequence identity with mouse and rat THSP4. Within the THSP type-3 repeats and the THSP C‑terminal domain, human THSP4 shares 79% aa sequence identity with THSP3 and COMP, and 58% aa sequence identity with THSP1 and THSP2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). THSP4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of THSP4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). THSP4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). THSP4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of THSP4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type THSP4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type THSP4, although the A387P variant induces a greater release of pro-inflammatory molecules (13).

  • References:
    1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
    2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
    3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
    4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
    5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
    6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
    7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
    8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
    9. Stenina, O.I. et al. (2003) Circulation 108:1514.
    10. Topol, E.J. et al. (2001) Circulation 104:2641.
    11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
    12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
    13. Pluskota, E. et al. (2005) Blood 106:3970.
  • Entrez Gene IDs:
    7060 (Human); 21828 (Mouse)
  • Alternate Names:
    THBS4; thrombospondin 4; Thrombospondin4; Thrombospondin-4; TSP4; TSP-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Thrombospondin-4 mediates TGF-?-induced angiogenesis
    Authors: S Muppala, R Xiao, I Krukovets, D Verbovetsk, R Yendamuri, N Habib, P Raman, E Plow, O Stenina-Ad
    Oncogene, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Thrombospondin expression in myofibers stabilizes muscle membranes
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Assessing the contribution of thrombospondin-4 induction and ATF6? activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction
    Sci Rep, 2016;6(0):32449.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Thrombospondin 4 deficiency in mouse impairs neuronal migration in the early postnatal and adult brain.
    Authors: Girard F, Eichenberger S, Celio M
    Mol Cell Neurosci, 2014;61(0):176-86.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013;497(7449):369-73.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.
    Authors: Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W
    Mod. Pathol., 2011;24(10):1390-403.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Increased cortical expression of two synaptogenic thrombospondins in human brain evolution.
    Authors: Caceres M, Suwyn C, Maddox M, Thomas JW, Preuss TM
    Cereb. Cortex, 2007;17(10):2312-21.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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