Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Primate - Pan troglodytes (Chimpanzee)

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Thrombospondin-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443

Specificity

Detects human Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Thrombospondin-4 is observed and less than 5% cross-reactivity with recombinant human (rh) Thrombospondin-1, rhThrombospondin-2, and rhThrombospondin-3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Thrombospondin‑4 Antibody

Detection of Human Thrombospondin-4 antibody by Western Blot.

Detection of Human Thrombospondin‑4 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Thrombospondin-4 antibody by Simple WesternTM.

Detection of Human Thrombospondin‑4 by Simple WesternTM.

Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse Thrombospondin-4 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Thrombospondin-4 by Immunocytochemistry/Immunofluorescence

TSP-4 promotes accumulation of macrophages in peritoneal tissue of mice with LPS-induced peritonitis.a The number of macrophages in peritoneal cavity in mice with LPS-induced peritonitis. *p < 0.05, n = 5. b TSP-4 expression in macrophages from the peritoneal cavity lavage (left panel) and in the peritoneal tissue. QRT-PCR, fold increase (RQ) over the values in control mice injected with PBS; n = 3; *p < 0.05. c Macrophages and TSP-4 in peritoneal tissue of WT and P387-TSP-4-KI mice with LPS-induced peritonitis. Immunofluorescence; blue = nuclei (DAPI), green = macrophages (anti-CD68), red = TSP-4 (anti-TSP-4). Scale bar is 20 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31974349), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Thrombospondin-4 by Immunocytochemistry/Immunofluorescence

Detection of Mouse Thrombospondin-4 by Immunocytochemistry/Immunofluorescence

LPS induces TSP-4 in cultured macrophages.a Cultured macrophage-like RAW264.7 and cells and mouse bone-marrow-derived macrophages (BMDM) were treated with LPS (0.5 μg/ml for 1–24 h); western blotting with anti-TSP-4 Ab. b Cultured RAW264.7 (upper panels) and BMDM (lower panels) treated with LPS for 24 h were stained with anti-TSP-4 Ab; green = TSP-4, blue = nuclei, DAPI. Scale bar is 10 µm. c RAW 264.7 and BMDM cells were treated with LPS (0.5 μg/mL) for 24 h, and qRT-PCR was done (control = PBS treated); d RAW 264.7 cells were treated with CHX (25 μM) for 1–5 h and CHX (25 μM) + LPS (0.5 μg/mL) for 1–3 h followed by western blot detection of TSP-4 ( beta -actin as loading controls). CHX was added at time 0, followed by addition of LPS. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31974349), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Thrombospondin‑4 Antibody

Application
Recommended Usage

Simple Western

2 µg/mL
Sample: Human heart tissue

Western Blot

0.2 µg/mL
Sample: Human heart tissue

Reviewed Applications

Read 6 reviews rated 4.3 using AF2390 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thrombospondin-4

Thrombospondin-4 (THSP4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. THSP1 and THSP2 constitute subgroup A and form disulfide-linked homotrimers, whereas THSP3, THSP4, and THSP5/COMP constitute subgroup B and form pentamers (1, 2). The human THSP4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven THSP type-3 repeats (one with an RGD motif), and a THSP C‑terminal domain (3). Human THSP4 shares 93% aa sequence identity with mouse and rat THSP4. Within the THSP type-3 repeats and the THSP C‑terminal domain, human THSP4 shares 79% aa sequence identity with THSP3 and COMP, and 58% aa sequence identity with THSP1 and THSP2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). THSP4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of THSP4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). THSP4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). THSP4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of THSP4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type THSP4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type THSP4, although the A387P variant induces a greater release of pro-inflammatory molecules (13).

References

  1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
  3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
  6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
  7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
  8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  9. Stenina, O.I. et al. (2003) Circulation 108:1514.
  10. Topol, E.J. et al. (2001) Circulation 104:2641.
  11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
  12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
  13. Pluskota, E. et al. (2005) Blood 106:3970.

Alternate Names

THBS4, Thrombospondin4, TSP-4

Entrez Gene IDs

7060 (Human); 21828 (Mouse)

Gene Symbol

THBS4

UniProt

P35443

Additional Thrombospondin-4 Products

Product Documents for Human Thrombospondin‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Thrombospondin‑4 Antibody

For research use only

Citations for Human Thrombospondin‑4 Antibody

Customer Reviews for Human Thrombospondin‑4 Antibody (6)

4.3 out of 5
6 Customer Ratings
5 Stars
50%
4 Stars
33%
3 Stars
17%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 of 6 reviews Showing All
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  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/01/2024
    Human Thrombospondin‑4 Antibody AF2390
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Thrombospondin‑4 Antibody AF2390
  • Human Thrombospondin-4 Antibody
    Name: Arul Subramanian
    Application: Immunohistochemistry
    Sample Tested: Embryo
    Species: Zebrafish
    Verified Customer | Posted 03/14/2020
    The antibody was used to detect human TSP4 protein (Red channel in the figure) that was injected into a zebrafish embryo. The antibody did not cross-react with zebrafish Tsp4 protein. The data has been published (eLife 2014;3:e02372).
    Human Thrombospondin‑4 Antibody AF2390
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA in combination with mAb (MAB2390)) and protein (2390-TH). This combination works very well for detecting the TSP4 in human serum and plasma
    Human Thrombospondin‑4 Antibody AF2390
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: Luminex
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 11/08/2018
  • Human Thrombospondin-4 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018

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Associated Pathways

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