Human Thrombospondin-4 Antibody
Human Thrombospondin-4 Antibody Summary
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Thrombospondin‑4 by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Thrombospondin-4 at approximately 130kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Thrombospondin‑4 by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin-4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Thrombospondin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombospondin-4
Thrombospondin-4 (THSP4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. THSP1 and THSP2 constitute subgroup A and form disulfide-linked homotrimers, whereas THSP3, THSP4, and THSP5/COMP constitute subgroup B and form pentamers (1, 2). The human THSP4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven THSP type-3 repeats (one with an RGD motif), and a THSP C‑terminal domain (3). Human THSP4 shares 93% aa sequence identity with mouse and rat THSP4. Within the THSP type-3 repeats and the THSP C‑terminal domain, human THSP4 shares 79% aa sequence identity with THSP3 and COMP, and 58% aa sequence identity with THSP1 and THSP2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). THSP4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of THSP4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). THSP4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). THSP4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of THSP4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type THSP4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type THSP4, although the A387P variant induces a greater release of pro-inflammatory molecules (13).
- Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
- Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
- Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
- Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
- Hauser, N. et al. (1995) FEBS Lett. 368:307.
- Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
- Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
- Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
- Stenina, O.I. et al. (2003) Circulation 108:1514.
- Topol, E.J. et al. (2001) Circulation 104:2641.
- Wessel, J. et al. (2004) Am. Heart J. 147:905.
- Stenina, O.I. et al. (2005) FASEB J. 19:1893.
- Pluskota, E. et al. (2005) Blood 106:3970.
Product Datasheets
Citations for Human Thrombospondin-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Thrombospondin-4 mediates TGF-?-induced angiogenesis
Authors: S Muppala, R Xiao, I Krukovets, D Verbovetsk, R Yendamuri, N Habib, P Raman, E Plow, O Stenina-Ad
Oncogene, 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Assessing the contribution of thrombospondin-4 induction and ATF6? activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction
Sci Rep, 2016;6(0):32449.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Thrombospondin expression in myofibers stabilizes muscle membranes
Elife, 2016;5(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Thrombospondin 4 deficiency in mouse impairs neuronal migration in the early postnatal and adult brain.
Authors: Girard F, Eichenberger S, Celio M
Mol Cell Neurosci, 2014;61(0):176-86.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
Nature, 2013;497(7449):369-73.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling.
Authors: Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W
Mod. Pathol., 2011;24(10):1390-403.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Increased cortical expression of two synaptogenic thrombospondins in human brain evolution.
Authors: Caceres M, Suwyn C, Maddox M, Thomas JW, Preuss TM
Cereb. Cortex, 2007;17(10):2312-21.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
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The antibody was used to detect human TSP4 protein (Red channel in the figure) that was injected into a zebrafish embryo. The antibody did not cross-react with zebrafish Tsp4 protein.
The data has been published (eLife 2014;3:e02372).
We used this antibody for a sandwich ELISA in combination with mAb (MAB2390)) and protein (2390-TH). This combination works very well for detecting the TSP4 in human serum and plasma
