Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 904802
View all Thyroglobulin Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Thyroglobulin
Met1-Lys2768
Accession # P01266

Specificity

Detects human Thyroglobulin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Thyroglobulin Antibody

Detection of Human Thyroglobulin antibody by Western Blot.

Detection of Human Thyroglobulin by Western Blot.

Western blot shows lysates of human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Thyroglobulin at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Thyroglobulin antibody in Human Thyroid Cancer Tissue by Immunohistochemistry (IHC-P).

Thyroglobulin in Human Thyroid Cancer Tissue.

Thyroglobulin was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Mouse Anti-Human Thyroglobulin Monoclonal Antibody (Catalog # MAB8306) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Thyroglobulin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thyroid cancer tissue

Western Blot

1 µg/mL
Sample: Human thyroid tissue

Reviewed Applications

Read 1 review rated 4 using MAB8306 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Thyroglobulin

Human Thyroglobulin (Tg) gene is localized at position 8q242-8q243. The gene covers a genome area of at least 300 000 bp and contains at least 37 exons. Thyroglobulin (Tg) is a dimeric 660 kDa glycoprotein produced by follicular cells (thyrocytes) in thyroid gland and stored in the follicular colloid of the gland. Tg is heterogenic due to post-translational modifications, such as iodination, glycosylation, sulfatation, phosphorylation and it is highly sensitivity to proteolysis.  Acting as a substrate, Tg is used for the synthesis of thyroid hormones thyroxine (T4) and triiodothyronine (T3).  Tg level can serve as a tumor marker of papillary and follicular thyroid cancer and elevated levels of anti-Tg antibodies in blood can be indicative of Graves disease. 

Alternate Names

AITD3, cog, TDH3, Tg, TGN

Entrez Gene IDs

7038 (Human); 21819 (Mouse); 24826 (Rat)

Gene Symbol

TG

UniProt

P01266

Additional Thyroglobulin Products

Product Documents for Human Thyroglobulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Thyroglobulin Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Customer Reviews for Human Thyroglobulin Antibody (1)

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  • Human Thyroglobulin Antibody
    Name: Christof Bertram
    Application: Immunohistochemistry-Paraffin
    Sample Tested: thyroid gland and thyroid follicular tumor
    Species: horse
    Verified Customer | Posted 01/26/2017
    Thyroid follicular neoplasm, horse, Human thyroglobulin Antibody, Dilution 1:100, pretreatment: microwave heating with citrate buffer for 12 minutes at 600 watts,
    Application: Immunohistochemistry Species: Horse Dilution: 1:100 Pretreatment for antigen retrieval: microwave heating with citrate buffer for 12 minutes at 600 watts Results: positive staining of follicular epithelium
    Human Thyroglobulin Antibody MAB8306

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Protocols

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FAQs for Human Thyroglobulin Antibody

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  • Q: What antigen retrieval method was used to generate the IHC staining of human thyroid cancer tissue with Anti-human Thyroglobulin Monoclonal Antibody, Catalog # MAB8306?

    A: Heat Induced Epitope Retreival (HIER) method was used in house with basic antigen retrieval (pH 9).

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