Human Tie-1 Antibody

Catalog # Availability Size / Price Qty
MAB619
MAB619-SP
Tie‑1 in HUVEC Human Cells.
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Product Details
Citations (2)
FAQs
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Human Tie-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tie-1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 88016
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie-1
Ala22-Gln760 (predicted)
Accession # P35590
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Tie‑1 Fc Chimera (Catalog # 619-TI)
Immunohistochemistry
8-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Tie-1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Tie‑1 in HUVEC Human Cells. Tie-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Tie‑1 antibody in Human Gastric Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Tie‑1 in Human Gastric Cancer Tissue. Tie-1 was detected in immersion fixed paraffin-embedded sections of human gastric cancer tissue using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the pyloric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Tie-1 antibody in Human Stomach by Immunohistochemistry (IHC-P). View Larger

Tie‑1 in Human Stomach. Tie-1 was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-1

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-1 cDNA encodes a 1138 amino acid (aa) precursor with a 24 aa putative signal peptide, a 735 aa extracellular domain and a 354 aa cytoplasmic domain. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

References
  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Sato, T.N. et al. (1995) Nature 376:70.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1
Entrez Gene IDs
7075 (Human); 21846 (Mouse)
Alternate Names
EC 2.7.10; EC 2.7.10.1; JTK14soluble TIE1 variant 3; receptor tyrosine kinase; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 5; Tie1; Tie-1; TIEsoluble TIE1 variant 4; tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; tyrosine-protein kinase receptor Tie-1

Product Datasheets

Citations for Human Tie-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Sulfated glycans engage the Ang-Tie pathway to regulate vascular development
    Authors: ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils
    Nat Chem Biol, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Spatial and temporal distribution of Tie-1 and Tie-2 during very early development of the human placenta.
    Authors: Kayisli UA, Cayli S, Seval Y, Tertemiz F, Huppertz B, Demir R
    Placenta, 2006;27(6):648-59.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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