Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Knockout Validated, ELISA Capture (Matched Antibody Pair)
Cited:
Antibody Array Development, ELISA Detection
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 127711
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Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2
Cys27-Pro220
Accession # P16035
Cys27-Pro220
Accession # P16035
Specificity
Detects human TIMP-2 in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human TIMP-2 Antibody (127711)
Western Blot Shows Human TIMP‑2 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and human TIMP-2 knockout HeLa human cervical epithelial carcinoma cell line (KO). PVDF membrane was probed with 4 µg/mL of Mouse Anti-Human TIMP‑2 Monoclonal Antibody (Catalog # MAB9711) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated) in the parental HeLa human cervical epithelial carcinoma cell line, but is not detectable in knockout HeLa human cervical epithelial carcinoma cell line. GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Human TIMP-2 ELISA Standard Curve
Recombinant Human TIMP‑2 (Catalog # 971-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑2 Monoclonal Antibody (Catalog # MAB9711) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human TIMP-2 Antibody (127711)
Application
Recommended Usage
Knockout Validated
TIMP‑2
is specifically detected in HeLa human cervical epithelial carcinoma parental
cell line but is not detectable in TIMP‑2 knockout HeLa human
cervical epithelial carcinoma cell line.
Human TIMP-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB9711 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TIMP-2
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
UniProt
Additional TIMP-2 Products
Product Documents for Human TIMP-2 Antibody (127711)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TIMP-2 Antibody (127711)
For research use only
Related Research Areas
Citations for Human TIMP-2 Antibody (127711)
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Customer Reviews for Human TIMP-2 Antibody (127711) (1)
5 out of 5
1 Customer Rating
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/16/2017This antibody was used as the capture molecule with BAF971 as the detection antibody in an ELISA built to measure TIMP-2.
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