Detects human and mouse TIMP‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human TIMP-4 is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human TIMP‑2 Cys27-Pro220 Accession # P16035
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize Recombinant Human TIMP-2 (0.143 µg/mL, Catalog # 971-TM) inhibition of Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902-MPN or 902-MP) cleavage of the fluorogenic peptide substrate Mca‑PLGL‑Dpa‑AR‑NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 2.6 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TIMP‑2 by Western Blot. Western blot shows Recombinant Human TIMP-2 Western Blot Standard Protein (Catalog # WBC023) and lysates of HeLa human cervical epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP‑2 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
TIMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human/Mouse TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Neutralization of TIMP‑2 Activity by Human TIMP‑2 Antibody.
Recombinant Human MMP‑2 (0.2 µg/mL, Catalog # 902‑MPN or 902‑MP) activity is measured in the presence of Recombinant Human/Mouse TIMP-2 (0.143 µg/mL, Catalog # 971‑TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF971). The ND50 is typically 2.6 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid-potentiating and cell growth promoting activities.
Tissue Inhibitors of Metalloproteinases 2
Entrez Gene IDs:
7077 (Human); 21858 (Mouse); 29543 (Rat)
CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP2; TIMP-2; Tissue inhibitor of metalloproteinases 2
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