Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE (1). TIMP-3 also uniquely shows high affinity for binding to the extracellular matrix (2). Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.
Human TIMP‑3 Antibody
R&D Systems | Catalog # MAB9731
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Porcine
Applications
Validated:
Intracellular Staining by Flow Cytometry, Immunoprecipitation, CyTOF-reported
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 277128
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-3
Cys24-Pro211
Accession # P35625
Cys24-Pro211
Accession # P35625
Specificity
Detects human TIMP-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TIMP-1, -2, or -4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human TIMP‑3 Antibody
Application
Recommended Usage
CyTOF-reported
Brodie, T.M. et al. (2018) Cytometry Part
A. 93: 406. Ready to be labeled using established
conjugation methods. No BSA or other carrier proteins that could interfere with
conjugation.
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TIMP‑3 (Catalog # 973-TM), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human TIMP‑3 (Catalog # 973-TM), see our available Western blot detection antibodies
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: MDA‑MBA‑231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Sample: MDA‑MBA‑231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TIMP-3
References
- Amour, A. et al. (1998) FEBS Lett. 435:39.
- Leco, K.J. et al. (1994) J. Biol. Chem. 269:9352.
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
UniProt
Additional TIMP-3 Products
Product Documents for Human TIMP‑3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human TIMP‑3 Antibody
For research use only
Related Research Areas
Citations for Human TIMP‑3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
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- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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