Human TNF-alpha (Research Grade Adalimumab Biosimilar) Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
MAB9677-100
MAB9677-SP
Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry.
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Product Details
Citations (1)
FAQs
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Human TNF-alpha (Research Grade Adalimumab Biosimilar) Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF- alpha in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu7
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human TNF‑ alpha
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 2-12 ng/mL in the presence of 0.25 ng/mL Recombinant Human TNF‑ alpha and actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of TNF- alpha antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) treated with PMA and Ca2+ ionomycin overnight, or (B) resting, were stained with Human Anti-Human TNF- alpha (Research Grade Adalimumab Biosimilar) Monoclonal Antibody (Catalog # MAB9677) followed by Goat anti-Human APC-conjugated Secondary Antibody (Catalog # F0135) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Neutralization Cytotoxicity Induced by TNF-alpha  and Neutralization by Human TNF-alpha  Antibody. View Larger

Cytotoxicity Induced by TNF-alpha and Neutralization by Human TNF-alpha Antibody. Recombinant Human TNF-a (Catalog # 210-TA) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Cytotoxicity elicited by Recombinant Human TNF-a (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human Anti-Human TNF-a (Research Grade Adalimumab Biosimilar) Monoclonal Antibody (Catalog # MAB9677). The ND50 is typically 2-12 ng/mL in the presence of the metabolic inhibitor actinomycin D.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Adalimumab is a biosimilar directed against the extracellular domain of TNF- alpha. This constitutes the immunogen used for the anti-Idiotype catalog numbers MAB9616 and MAB9546. The biotinylated antibody (catalog number IC9677B) can be used in flow cytometry or various assay format to measure EGFR expression and can be detected by conjugated streptavidin.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Product Datasheets

Citation for Human TNF-alpha (Research Grade Adalimumab Biosimilar) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Population-wide diversity and stability of serum antibody epitope repertoires against human microbiota
    Authors: T Vogl, S Klompus, S Leviatan, IN Kalka, A Weinberger, C Wijmenga, J Fu, A Zhernakova, RK Weersma, E Segal
    Nature Medicine, 2021;0(0):.
    Species: Staphylococcus
    Sample Types:
    Applications: Neutralization Control

FAQs

  1. What is a Biosimilar antibody?

    • A Biosimilar antibody is a non-therpeutic, recombinantly-expressed antibody which has the same sequence as the corresponding therapeutic antibody.  It is for research use only. The nomenclature of the Biosimilar antibody includes the name of the therapeutic antibody.  For example, Catalog # MAB9577 is called Human EGFR (Research Grade CetuxiMab Biosimilar) antibody.

  2. Does this Biosimilar antibody have the same sequence as its corresponding therapeutic antibody? 

    • This Biosimilar antibody has the same variable region sequence as the therapeutic antibody, but the constant region is not the same. It contains a proprietary human IgG constant region. 

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