Human TNF-alpha (Research Grade Adalimumab Biosimilar) Biotinylated Antibody

Recombinant Monoclonal Antibody For Research Use Only
Catalog # Availability Size / Price Qty
IC9677B-100
Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry.
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Human TNF-alpha (Research Grade Adalimumab Biosimilar) Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TNF- alpha in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Adalimumab. This product is for research use only.
Source
Recombinant Monoclonal Human IgG1 Clone # Hu7
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Human TNF‑ alpha
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Biotin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of TNF- alpha antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of TNF‑ alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) (A) treated with PMA and Ca2+ ionomycin overnight or (B) resting, were stained with Human Anti-Human TNF- alpha (Research Grade Adalimumab Biosimilar) Biotinylated Monoclonal Antibody (Catalog # FAB9677B) followed by APC-conjugated Streptavidin (Catalog # F0050) and Mouse anti-Human CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB100P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TNF-alpha

Adalimumab is a biosimilar directed against the extracellular domain of TNF- alpha. The biotinylated antibody can be used in flow cytometry or various assay format to measure TNF- alpha expression and can be detected by conjugated streptavidin.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

Product Datasheets

FAQs

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