Human TRAF-6 Antibody

Catalog # Availability Size / Price Qty
AF3284
AF3284-SP
Human TRAF-6 Antibody in Data
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human TRAF-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRAF-6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -4, and -5 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human TRAF-6
Met1-Val522
Accession # Q9Y4K3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human TRAF‑6 antibody by Western Blot. View Larger

Detection of Human TRAF‑6 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, -TRAF5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-6 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-6

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events.

Long Name
TNF Receptor-Associated Factor 6
Entrez Gene IDs
7189 (Human); 22034 (Mouse)
Alternate Names
E3 ubiquitin-protein ligase TRAF6; EC 6.3.2.-; Interleukin-1 signal transducer; RING finger protein 85; RNF85MGC:3310; TNF receptor-associated factor 6; TRAF6; TRAF-6

Product Datasheets

Citations for Human TRAF-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
    Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
    Chin. Med. J., 2020;0(6):679-686.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  2. USP17 Suppresses Tumorigenesis and Tumor Growth through Deubiquitinating AEP
    Authors: X Chen, C Wang, K Liao, S Zhou, L Cao, J Chen, C Xu, Y Lin
    Int. J. Biol. Sci., 2019;15(4):738-748.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human TRAF-6 Antibody

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Human TRAF-6 Antibody
By Anonymous on 06/07/2018
Application: IHC Sample Tested: Colon tissue Species: Human