Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRAF-6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TRAF-6
Met1-Val522
Accession # Q9Y4K3

Specificity

Detects human TRAF-6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-1, -2, -3, -4, and -5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human TRAF‑6 Antibody

Detection of Human TRAF‑6 antibody by Western Blot.

Detection of Human TRAF‑6 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human TRAF-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, -TRAF5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-6 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human TRAF‑6 by Simple WesternTM.

Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.2 mg/mL. A specific band was detected for TRAF‑6 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human TRAF‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3284). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human TRAF‑6 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: HEK293T human embryonic kidney cell line

Western Blot

1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 1 review rated 4 using AF3284 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAF-6

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL‑1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-6 is a 522 amino acid, 60 kDa protein. Overexpression of TRAF-6 mediates the activation of NF-kappa-B and JNK signaling pathways. TRAF-3 and TRAF-6 have been identified as adaptor molecules involved in TLR/IL-1R signaling events.

Long Name

TNF Receptor-Associated Factor 6

Alternate Names

TRAF6

Entrez Gene IDs

7189 (Human); 22034 (Mouse)

Gene Symbol

TRAF6

UniProt

Q9Y4K3

Additional TRAF-6 Products

Product Documents for Human TRAF‑6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TRAF‑6 Antibody

For research use only

Citations for Human TRAF‑6 Antibody

Customer Reviews for Human TRAF‑6 Antibody (1)

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  • Human TRAF-6 Antibody
    Name: Christian Ostalecki
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Human
    Verified Customer | Posted 06/07/2018
    Human TRAF‑6 Antibody AF3284

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