Human TRANCE/TNFSF11/RANK L Antibody

Catalog # Availability Size / Price Qty
AB626
Osteoclast-like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.
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Human TRANCE/TNFSF11/RANK L Antibody Summary

Species Reactivity
Human
Specificity
Detects TRANCE/TNFSF11/RANK L in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse TRANCE is observed.    
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRANCE/TNFSF11/RANK L
Gln73-Asp317 (Ala194Gly)
Accession # O14788
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TRANCE/TNFSF11/RANK L (Truncated) (Catalog # 390-TN)
Neutralization
Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse splenocytes. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND50) is typically 5-15 µg/mL in the presence of 30 ng/mL Recombinant Human TRANCE/TNFSF11/RANK L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Osteoclast-like Cell Formation Induced by TRANCE/TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody. View Larger

Osteoclast-like Cell Formation Induced by TRANCE/
TNFSF11/RANK L and Neutralization by Human TRANCE/TNFSF11/RANK L Antibody.
In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/TNFSF11/
RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TRANCE/
TNFSF11/RANK L Polyclonal Antibody (Catalog # AB626). The ND50 is typically 5‑15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

RANK Ligand (receptor activator of NF-kappa B ligand [RANKL], also called TNF-related activation-induced cytokines (TRANCE), osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. RANK Ligand was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The human RANK Ligand cDNA encodes a type II transmembrane protein of 317 amino acids with a predicted cytoplasmic domain of 47 amino acids, a 21 amino acids transmembrane region, and an extracellular domain of 249 amino acids. The extracellular domain contains two potential N‑linked glycosylation sites. Mouse and human RANK Ligand share 85% amino acid identity. RANK Ligand is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of RANK Ligand include induction of activation of the c-jun N‑terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of RANK Ligand. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds RANK Ligand, is a naturally occurring decoy receptor that counterbalances the effects of RANK Ligand.

References
  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.
Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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