Human Transgelin/TAGLN Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Transgelin/TAGLN in ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 859112
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Transgelin/TAGLN
    Ala2-Ser201
    Accession # Q01995
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human and Rat Transgelin/TAGLN by Western Blot. Western blot shows lysates of human aorta tissue, human uterus tissue, and rat uterus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Transgelin/TAGLN Monoclonal Antibody (Catalog # MAB78861) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Transgelin/TAGLN at approximately 16-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry
Transgelin/TAGLN in MCF 10A Human Cell Line. Transgelin/TAGLN was detected in immersion fixed MCF 10A human breast epithelial cell line using Mouse Anti-Human Transgelin/TAGLN Monoclonal Antibody (Catalog # MAB78861) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Transgelin/TAGLN by Simple WesternTM. Simple Western lane view shows lysates of human aorta tissue and human uterus tissue, loaded at 0.5 mg/mL. A specific band was detected for Transgelin/TAGLN at approximately 22-25 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Transgelin/TAGLN Monoclonal Antibody (Catalog # MAB78861). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Transgelin/TAGLN

TAGLN (Transgelin; also 22 kDa Actin-binding protein, WS3-10 and Smooth muscle protein 22 alpha) is a 22-24 kDa cytosolic member of the calponin (calcium-binding and calmodulin-binding troponin T-like protein) family of molecules. It is expressed in both visceral and vascular smooth muscle, fibroblasts, cardiac myocytes, and potentially in breast duct plus prostate epithelium. TAGLN is associated with the actin stress fibers and appears to both suppress MMP-9 production, and downmodulate Ca++-independent smooth muscle contraction. Human TAGLN is 201 amino acids (aa) in length. It contains one CH/calponin homology domain (aa 24-137), and an actin-binding calponin-like repeat/CLIK (aa 175-200). There is one utilized phosphorylation site at Tyr193, plus two utilized acetylation sites in the N-terminus. Three potential isoform variants are reported, one that shows a alternative start site at Met111, a second that contains a new start site 10 aa upstream of the standard site, and a third that possesses a 58 aa substitution for aa 97-201. Full-length human and mouse TAGLN share 97% aa sequence identity.

  • Entrez Gene IDs:
    6876 (Human); 21345 (Mouse); 25123 (Rat)
  • Alternate Names:
    DKFZp686P11128; Protein WS3-10; SM22 alpha; SM2222 kDa actin-binding protein; SMCCDKFZp686B01212; Smooth muscle protein 22-alpha; TAGLN; TAGLN1; transgelin variant 2; Transgelin; WS3-10; WS3-10SM22-alpha
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