Human Transglutaminase 2/TGM2 Antibody

Catalog # Availability Size / Price Qty
MAB4376
MAB4376-SP
Detection of Human Transglutaminase 2/TGM2 by Western Blot.
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Human Transglutaminase 2/TGM2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Transglutaminase 2/TGM2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Transglutaminase 3, 4, 7, or recombinant mouse Transglutaminase 2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 716620
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 2/TGM2
Ala2-Ala687
Accession # P21980
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Transglutaminase 2/TGM2 by Western Blot. View Larger

Detection of Human Transglutaminase 2/TGM2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Transglutaminase 2/TGM2 Monoclonal Antibody (Catalog # MAB4376) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Transglutaminase 2/TGM2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Transglutaminase 2/TGM2

Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein-glutamine-gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6‑7).

References
  1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  2. Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
  3. Griffin, M. et al. (2002) Biochem. J. 368:377.
  4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
  5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
  6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
  7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.
Entrez Gene IDs
7052 (Human); 21817 (Mouse); 56083 (Rat)
Alternate Names
C polypeptide; EC 2.3.2.13; G-ALPHA-h; protein-glutamine gamma-glutamyltransferase 2; protein-glutamine-gamma-glutamyltransferase; TG(C); TG2; TGase C; TGase H; TGase-2; TGase-H; TGC; TGCGNAH; TGM2; Tissue transglutaminase; transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase); Transglutaminase 2; Transglutaminase C; Transglutaminase H; transglutaminase-2; tTG

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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