hh Human Trappin-2/Elafin Antibody AF1747: R&D Systems

Human Trappin-2/Elafin Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Trappin‑2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM-2, and human Neutrophil Elastase is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Trappin‑2/Elafin
    Ala23-Gln117
    Accession # P19957
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Trappin‑2/Elafin (Catalog # 1747-PI)
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human Trappin‑2/Elafin (Catalog # 1747-PI), see our available Western blot detection antibodies
    • Human Trappin-2/Elafin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human Trappin‑2/Elafin Antibody (Catalog # AF1747)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human Trappin‑2/Elafin Biotinylated Antibody (Catalog # BAF1747)
  • ELISA Standard
     
    Recombinant Human Trappin-2/Elafin Protein, CF (Catalog # 1747-PI)
  • Neutralization
    Measured by its ability to neutralize Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) inhibition of Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) cleavage of the fluorogenic peptide substrate MeOSuc-AAPV-Amc (100 µM). The Neutralization Dose (ND50) is typically 15.6 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Neutralization of Trappin‑2/
Elafin Activity by Human Trappin‑2/Elafin Antibody.
Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747‑PI) that has been pre­incubated with increasing concentrations of Goat Anti-Human Trappin‑2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND50 is typically 15.6 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trappin-2/Elafin

Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.

  • References:
    1. Schalkwijk, J. et al. (1999) Biochem. J. 340:569.
  • Long Name:
    Elastase Inhibitor, Transglutaminase Substrate, Serine Protease
  • Entrez Gene IDs:
    5266 (Human)
  • Alternate Names:
    cementoin; Elafin; Elastase-specific inhibitor; ESI; ESIWAP four-disulfide core domain protein 14; Peptidase inhibitor 3; peptidase inhibitor 3, skin-derived; PI3; PI-3; pre-elafin; protease inhibitor 3, skin-derived (SKALP); Protease inhibitor WAP3; SKALP; SKALPELAFIN; Skin-derived antileukoproteinase; Trappin2; Trappin-2; WAP four-disulfide core domain 14; WAP3MGC13613; WFDC14elafin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression.
    Authors: Burgener, Adam, Tjernlund, Annelie, Kaldensjo, Tove, Abou, Max, McCorrister, Stuart, Westmacott, Garrett, Mogk, Kenzie, Ambrose, Emma, Broliden, Kristina, Ball, Blake
    J Virol, 2013;87(9):5141-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Anti-HIV-1 activity of elafin is more potent than its precursor's, trappin-2, in genital epithelial cells.
    Authors: Drannik AG, Nag K, Yao XD, Henrick BM, Jain S, Ball TB, Plummer FA, Wachihi C, Kimani J, Rosenthal KL
    J. Virol., 2012;86(8):4599-610.
    Species: Human
    Sample Type: Vaginal Lavage
    Application: Immunodepletion
  3. Elafin, an Elastase-specific Inhibitor, Is Cleaved by Its Cognate Enzyme Neutrophil Elastase in Sputum from Individuals with Cystic Fibrosis.
    Authors: Guyot N, Butler MW, McNally P, Weldon S, Greene CM, Levine RL, O'Neill SJ, Taggart CC, McElvaney NG
    J. Biol. Chem., 2008;283(47):32377-85.
    Species: Human
    Sample Type: Sputum
    Application: ELISA Development
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