Detects human Trappin‑2/Elafin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) SLPI, rhTGM-2, and human Neutrophil Elastase is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Trappin‑2/Elafin Ala23-Gln117 Accession # P19957
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human Trappin‑2/Elafin (Catalog # 1747-PI)
Human Trappin‑2/Elafin Antibody (Catalog # AF1747)
ELISA Detection (Matched Antibody Pair)
Human Trappin‑2/Elafin Biotinylated Antibody (Catalog # BAF1747)
Recombinant Human Trappin-2/Elafin Protein, CF (Catalog # 1747-PI)
Measured by its ability to neutralize Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747-PI) inhibition of Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) cleavage of the fluorogenic peptide substrate MeOSuc-AAPV-Amc (100 µM). The Neutralization Dose (ND50) is typically 15.6 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Neutralization of Trappin‑2/ Elafin Activity by Human Trappin‑2/Elafin Antibody. Recombinant Mouse Neutrophil Elastase/ELA2 (1 µg/mL, Catalog # 4517-SE) activity is measured in the presence of Recombinant Human Trappin-2/ Elafin (1 µg/mL, Catalog # 1747‑PI) that has been preincubated with increasing concentrations of Goat Anti-Human Trappin‑2/Elafin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1747). The ND50 is typically 15.6 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Trappin-2 is the human member of the trappin gene family that contains SLPI (1). Trappin-2 consists of an N-terminal transglutaminase substrate domain (residues 23‑60) and a C-terminal four-disulfide core or whey acidic protein (WAP) domain (residues 72‑117). Elafin or ESI (elastase-specific inhibitor) and SKALP (skin-derived anti-leucoproteinase) are alternative names for Trappin-2 and reflect its protease targets. However, elafin and SKALP sometimes correspond only to the processed form that contains the C-terminal WAP domain of the molecule, which can be isolated naturally. The recombinant human Trappin-2 corresponds to the full‑length form (residues 23‑117), which migrates as two protein bands under SDS-PAGE due to an unidentified mechanism. In addition to its ability to inhibit human neutrophil elastase, it can also be used as a substrate for transglutaminases.
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