Human TRIM21 Antibody

Catalog # Availability Size / Price Qty
MAB62191
MAB62191-SP
Detection of Human TRIM21 by Western Blot.
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Human TRIM21 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TRIM21 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIM5 or rhTRIM32 is observed.
Source
Monoclonal Mouse IgG2A Clone # 672714
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human TRIM21
Arg195-Pro293
Accession # P19474
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TRIM21 antibody by Western Blot. View Larger

Detection of Human TRIM21 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB62191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TRIM21 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRIM21

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 16-55), a B-Box type zinc-finger region (aa 92-123), a coiled-coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.

Long Name
Tripartite Motif-containing Protein 21
Entrez Gene IDs
6737 (Human); 20821 (Mouse); 308901 (Rat)
Alternate Names
52 kDa ribonucleoprotein autoantigen Ro/SS-A; 52 kDa Ro/SSA; E3 ubiquitin-protein ligase TRIM21; EC 6.3.2.-; rhTRIM21; RNF81; RNF81RING finger protein 81; ro(SS-A); Ro52; RO52SS-A; Sicca syndrome antigen A; Sjoegren syndrome type A antigen; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); SSA1; SSA1SSA; TRIM21; tripartite motif containing 21,52 kDa Ro protein; tripartite motif-containing 21; Tripartite motif-containing protein 21

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