Human TrkA Antibody (994307)

R&D Systems | Catalog # MAB1752

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Key Product Details

Species Reactivity

Human

Applications

Neutralization, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 994307
View all TrkA Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TrkA
Ala33-Glu407
Accession # P04629

Specificity

Detects human TrkA in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human TrkA Antibody (994307)

Detection of TrkA antibody in HEK293 Human Cell Line Transfected with Human TrkA and eGFP antibody by Flow Cytometry.

Detection of TrkA in HEK293 Human Cell Line Transfected with Human TrkA and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human TrkA and eGFP was stained with (A) Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1752) or (B) Mouse IgG1 isotype control antibody (Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
TrkA antibody in C6 Rat Cell Line by Immunocytochemistry (ICC).

TrkA in C6 Rat Cell Line.

TrkA was detected in immersion fixed C6 rat glioma cell line transfected with TrkA (positive stain) or wildtype (negative stain) using Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1752) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cell Proliferation Induced by beta -NGF and Neutralization by Human TrkA Antibody.

Cell Proliferation Induced by beta -NGF and Neutralization by Human TrkA Antibody.

Recombinant Human beta -NGF (Catalog # 256-GF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human beta -NGF (2 ng/mL) is neutralized (green line) by increasing concent-rations of Mouse Anti-Human TrkA Monoclonal Antibody (Catalog # MAB1752). The ND50 is typically 5-40 ng/mL.

Applications for Human TrkA Antibody (994307)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human TrkA and eGFP

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C6 rat glioma cell line, wildtype and transfected with TrkA

Neutralization

Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 5-40 ng/mL in the presence of 2 ng/mL Recombinant Human beta ‑NGF.

Reviewed Applications

Read 1 review rated 4 using MAB1752 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TrkA

Trk A, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. Trk A, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct Trk A isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer Trk A isoform is the only isoform expressed within neuronal tissues whereas the shorter Trk A is expressed mainly in non-neuronal tissues. NGF binds to Trk A with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of Trk A with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.

References

  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Entrez Gene IDs

4914 (Human); 18211 (Mouse); 59109 (Rat); 102129364 (Cynomolgus Monkey)

Gene Symbol

NTRK1

UniProt

P04629

Additional TrkA Products

Product Documents for Human TrkA Antibody (994307)

Certificate of Analysis

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Product Specific Notices for Human TrkA Antibody (994307)

For research use only

Related Research Areas

Receptor Tyrosine Kinases (RTKs)

Customer Reviews for Human TrkA Antibody (994307) (1)

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  • Human TrkA Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human TrkA Antibody (994307) MAB1752

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