Detects human TSG-6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 70% cross-reactivity with recombinant mouse TSG-6 is observed and less than 2% cross-reactivity with recombinant human TSG-14 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human TSG-6 Trp18-Leu277 Accession # P98066
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
TSG‑6 in human PBMCs.
TSG‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human TSG‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2104) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TSG-6 (TNF-stimulated Gene 6), also known as TNFIP6 is a secreted, 35‑39 kDa group A member of the LINK-Module superfamily of proteins (1‑4). Human TSG-6 is synthesized as a 277 amino acid (aa) precursor. It contains a 17 aa signal sequence and a 260 aa mature region (5, 6). The mature region shows an N-terminal LINK module (amino acids 36‑129) and a C-terminal CUB (C1s/C1r; urchin embryonic growth factor; BMP1) domain (amino acids 135‑247). Link modules are alpha -helical, beta ‑sheet structures that bind hyaluronan (HA) and participate in extracellular matrix (ECM) assembly (7). Mature human TSG-6 shares 94% aa identity with both mouse and canine TSG-6. Cells reported to express TSG-6 include activated fibroblasts, synoviocytes, chondrocytes, neutrophils, proximal tubular epithelium, bronchial epithelium, endothelium, and visceral, plus vascular smooth muscle (2, 8). TSG-6 has multiple functions, many of which involve the ECM. It is suggested to stabilize HA-rich ECM. It does so by serving as an intermediary, or link, between the individual subunits of extracellular decameric pentraxin 3 and the surrounding hyaluronan matrix (9). It also provides structure and organization to hyaluronan. This is accomplished by a TSG-6 mediated transfer of an 80‑85 kDa HC subunit from I alpha I (inter‑ alpha ‑inhibitor) to HA. I alpha I is a four-component, 225 kDa serine protease inhibitor. It contains a protease inhibitor subunit (bikunin), two independent, accompaning protein chains (HC1 and HC2), and a short chondroitin sulfate linking moiety. TSG-6 is a cation-dependent catalyst for the removal, transfer, and subsequent covalent linkage of HC 1/2 to surrounding HA. This provides substance and reinforcement to the ECM (1, 2, 10‑12). The disassembly of I alpha I also leads to free bikunin, which in the “free” state becomes a potent inhibitor of serine proteases (8).
Milner, C.M. et al. (2006) Biochem. Soc. Trans. 34:446.
Milner, C.M. and A.J. Day (2003) J. Cell Sci. 116:1863.
Wisnieewski, H-G. and J. Vilcek (2004) Cytokine Growth Factor Rev. 15:129.
Blundell, C.D. et al. (2005) J. Biol. Chem. 280:18189.
Lee, T.H. et al. (1990) Mol. Cell. Biol. 10:1982.
Lee, T.H. et al. (1992) J. Cell Biol. 116:545.
Kohda, D. et al. (1996) Cell 86:767.
Forteza, R. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:20.
Salustri, A. et al. (2003) Development 131:1577.
Rugg, M.S. et al. (2005) J. Biol. Chem. 280:25674.
Sanggaard, K.W. et al. (2006) Biochemistry 45:7661.
Sanggaard, K.W. et al. (2005) J. Biol. Chem. 280:11936.
Tumor Necrosis Factor-stimulated Gene Sequence 6
Entrez Gene IDs:
7130 (Human); 21930 (Mouse)
Hyaluronate-binding protein; TNF alpha-induced protein 6; TNFAIP6; TNF-stimulated gene 6 protein; TSG6; TSG-6; TSG-6tumor necrosis factor alpha-inducible protein 6; TSG6tumor necrosis factor-inducible gene 6 protein; Tumor necrosis factor alpha-induced protein 6; tumor necrosis factor, alpha-induced protein 6; tumor necrosis factor-stimulated gene-6 protein
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