Human TSLP Antibody Summary
Accession # Q969D9
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human TSLP by Western Blot. Western blot shows lysates of human lung and kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB1398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1-3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.
- Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Pandey, A. et al. (2000) Nature Immunol. 1:59.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Quentmeier, H. et al. (2001) Leukemia 15:1286.
Citation for Human TSLP Antibody
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1 Citation: Showing 1 - 1
Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction.
Authors: Colletti E, Lindstedt S, Park PJ, Almeida-Porada G, Porada CD
Exp. Hematol., 2008;36(7):816-22.
Sample Types: Whole Tissue
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We used this antibody in an in-house ELISA along with pAb (AF1398) and protein (1398-TS-010) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.