Human TSLP Antibody Summary
Tyr29-Gln159
Accession # Q969D9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TSLP by Western Blot. Western blot shows lysates of human lung and kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB1398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.
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Detection of TSLP by Western Blot Cytokine production induced by co-stimulation with TWEAK and TGF-beta 1 were steroid unresponsiveness. The mRNA levels of TSLP (A,C), CCL5 (D), and CCL17 (F) after 48 h of treatment and CCL2 (H) and IL-8 (J) after 2 h of treatment, analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. TSLP levels after 48 h of treatment assessed by immunoblotting (B, upper panel). The density of each band quantified by densitometry using ImageJ (version 6.1) (B, lower). The levels of CCL5 (E) and CCL17 (G) after 48 h of treatment, and CCL2 (I) and IL-8 (K) after 2 h of treatment in cell culture supernatants, analyzed by enzyme-linked immunosorbent assay (ELISA). Data represent the mean ± SD of three independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/21/11625), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of TSLP by Western Blot MKP-1 is involved in steroid unresponsiveness induced by co-stimulation with TWEAK and TGF-beta 1 in BEAS-2B cells. The mRNA levels of MKP-1 (A), TSLP (C), and CCL5 (E), analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. MKP-1 and thymic stromal lymphopoietin (TSLP) expression after 48 h of treatment, assessed by immunoblotting (B,D, upper panel). Densitometric analysis of protein bands (B,D, lower). The level of CCL5 in cell culture supernatants after 48 h of treatment, analyzed using ELISA (F). Data represent mean ± SD of two independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX; ‡ p < 0.05 compared with control siRNA. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/21/11625), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1-3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.
- Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
- Park, L.S. et al. (2000) J. Exp. Med. 192:659.
- Pandey, A. et al. (2000) Nature Immunol. 1:59.
- Reche, P.A. et al. (2001) J. Immunol. 167:336.
- Quentmeier, H. et al. (2001) Leukemia 15:1286.
Product Datasheets
Citations for Human TSLP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Co-Stimulation with TWEAK and TGF-?1 Induces Steroid-Insensitive TSLP and CCL5 Production in BEAS-2B Human Bronchial Epithelial Cells
Authors: Abe, S;Harada, N;Sandhu, Y;Sasano, H;Tanabe, Y;Ueda, S;Nishimaki, T;Sato, Y;Takeshige, T;Harada, S;Akiba, H;Takahashi, K;
International journal of molecular sciences
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction.
Authors: Colletti E, Lindstedt S, Park PJ, Almeida-Porada G, Porada CD
Exp. Hematol., 2008-04-08;36(7):816-22.
Species: Ovine
Sample Types: Whole Tissue
Applications: IHC-Fr
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We used this antibody in an in-house ELISA along with pAb (AF1398) and protein (1398-TS-010) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.
