Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Ovine

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 258136
View all TSLP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TSLP
Tyr29-Gln159
Accession # Q969D9

Specificity

Detects human TSLP in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑7 or rmTSLP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human TSLP Antibody

Detection of Human TSLP antibody by Western Blot.

Detection of Human TSLP by Western Blot.

Western blot shows lysates of human lung and kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TSLP Monoclonal Antibody (Catalog # MAB1398) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSLP at approximately 18 kDa (as indicated). This experiment was conducted under non-reducing conditions only and using Immunoblot Buffer Group 1.

Detection of TSLP by Western Blot

Detection of TSLP by Western Blot

Cytokine production induced by co-stimulation with TWEAK and TGF-beta 1 were steroid unresponsiveness. The mRNA levels of TSLP (A,C), CCL5 (D), and CCL17 (F) after 48 h of treatment and CCL2 (H) and IL-8 (J) after 2 h of treatment, analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. TSLP levels after 48 h of treatment assessed by immunoblotting (B, upper panel). The density of each band quantified by densitometry using ImageJ (version 6.1) (B, lower). The levels of CCL5 (E) and CCL17 (G) after 48 h of treatment, and CCL2 (I) and IL-8 (K) after 2 h of treatment in cell culture supernatants, analyzed by enzyme-linked immunosorbent assay (ELISA). Data represent the mean ± SD of three independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/21/11625), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of TSLP by Western Blot

Detection of TSLP by Western Blot

MKP-1 is involved in steroid unresponsiveness induced by co-stimulation with TWEAK and TGF-beta 1 in BEAS-2B cells. The mRNA levels of MKP-1 (A), TSLP (C), and CCL5 (E), analyzed by qRT–PCR. Data represent mean ± SD of two independent experiments. MKP-1 and thymic stromal lymphopoietin (TSLP) expression after 48 h of treatment, assessed by immunoblotting (B,D, upper panel). Densitometric analysis of protein bands (B,D, lower). The level of CCL5 in cell culture supernatants after 48 h of treatment, analyzed using ELISA (F). Data represent mean ± SD of two independent experiments. * p < 0.05, compared to untreated cultures as controls; † p < 0.05 compared with cultures in the absence of DEX; ‡ p < 0.05 compared with control siRNA. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/25/21/11625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human TSLP Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human lung and kidney tissue under non-reducing conditions only

Reviewed Applications

Read 1 review rated 3 using MAB1398 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSLP

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1-3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

References

  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  5. Quentmeier, H. et al. (2001) Leukemia 15:1286.

Long Name

Thymic Stromal Lymphopoietin

Alternate Names

thymic stromal lymphopoietin

Entrez Gene IDs

85480 (Human); 53603 (Mouse); 102119254 (Cynomolgus Monkey)

Gene Symbol

TSLP

UniProt

Q969D9

Additional TSLP Products

Product Documents for Human TSLP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human TSLP Antibody

For research use only

Citations for Human TSLP Antibody

Customer Reviews for Human TSLP Antibody (1)

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  • Human TSLP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/09/2020
    We used this antibody in an in-house ELISA along with pAb (AF1398) and protein (1398-TS-010) to quantify TSLP in human serum and plasma. This combination could not detect TSLP samples but generated a good standard curve.
    Human TSLP Antibody MAB1398

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