Detection of TSLP R in Human PBMC Monocytes by Flow Cytometry. Human PBMC monocytes were stained with Human TSLP R Monoclonal Antibody (Catalog # MAB981) followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B) and Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F). Quadrant markers were set based on control antibody staining (Catalog # MAB002).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TSLP R
TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The human TSLP R cDNA encodes a 371 amino acid (aa) residue type 1 membrane protein with a 22 aa residue signal peptide, a 210 aa residue extracellular domain, a 20 aa residue transmembrane domain, and a 119 aa residue cytoplasmic domain (4, 5). The extracellular region contains two fibronectin type III-like domains and a WSXWS-like motif. The cytoplasmic domain contains a membrane-proximal box 1 motif that is known to be important for association with JAKs (4). Human TSLP R displays 39% identity to mouse TSLP R and 24% identity to the common gamma receptor (4). An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported in mouse (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3‑6). The TSLP R and IL-7 R alpha are co‑expressed primarily on monocytes and dendritic cells and at lower levels in lymphoid cells. TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (5).
Fujio, K. et al. (2000) Blood 95:2204.
Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
Park, L.S. et al. (2000) J. Exp. Med. 192:659.
Tonozuka, Y. et al. (2001) Cytogenet. Cell Genet. 93:23.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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