Human Tyro3/Dtk Antibody

Catalog # Availability Size / Price Qty
AF859
AF859-SP
Detection of Human Tyro3/Dtk by Western Blot.
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Product Details
Citations (6)
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Human Tyro3/Dtk Antibody Summary

Species Reactivity
Human
Specificity
Detects human Tyro3/Dtk in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Dtk is observed and less than 1% cross-reactivity with recombinant human Axl, rmAxl, and rmMer is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Tyro3/Dtk
Ala41-Ser428
Accession # Q06418
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Tyro3/Dtk antibody by Western Blot. View Larger

Detection of Human Tyro3/Dtk by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, U-87 MG human glioblastoma/astrocytoma cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF859) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Tyro3/Dtk at approximately 97 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Tyrosine Protein Kinase Receptor TYRO3
Entrez Gene IDs
7301 (Human); 22174 (Mouse)
Alternate Names
Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY

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Citations for Human Tyro3/Dtk Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein
    Authors: H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim
    Nature Medicine, 2022;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Monitoring Viral Entry in Real-Time Using a Luciferase Recombinant Vesicular Stomatitis Virus Producing SARS-CoV-2, EBOV, LASV, CHIKV, and VSV Glycoproteins
    Authors: MF Lay Mendoz, MD Acciani, CN Levit, C Santa Mari, MA Brindley
    Viruses, 2020;12(12):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy
    Authors: K Kongmanas, N Punyadee, K Wasuworawo, A Songjaeng, T Prommool, Y Pewkliang, S Manocheewa, S Thiemmeca, K Sa-Ngiamsu, C Puttikhunt, KF Faull, S Hongeng, P Avirutnan
    PLoS Negl Trop Dis, 2020;14(11):e0008835.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Human Sertoli cells support high levels of Zika virus replication and persistence
    Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
    Sci Rep, 2018;8(1):5477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma
    Authors: C Dantas-Bar, T Lesluyes, FL Loarer, F Chibon, I Treilleux, JM Coindre, P Meeus, M Brahmi, O Bally, I Ray-Coquar, MP Sunyach, AL Cesne, O Mir, S Bonvalot, M Toulmonde, A Italiano, P Saintigny, M Jean-Denis, F Ducimetier, D Ranchere, H El Sayadi, L Alberti, JY Blay
    Br. J. Cancer, 2017;117(12):1787-1797.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. The Gas6-Axl Protein Interaction Mediates Endothelial Uptake of Platelet Microparticles
    Authors: KE Happonen, S Tran, M Mörgelin, R Prince, S Calzavarin, A Angelillo-, B Dahlbäck
    J. Biol. Chem., 2016;291(20):10586-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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