Human VEGFR2/KDR/Flk-1 Antibody

Name: Human VEGFR2/KDR/Flk-1 Antibody
Brand: R&D Systems
SKU: MAB3572-500
Rating: 5 (1 reviews)

MAB3572 17 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3572-SP
MAB3572-100
MAB3572-500

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Citations (17)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human VEGFR2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3 or recombinant mouse VEGFR2 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 89106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # P35968

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Neutralization

Measured by its ability to neutralize VEGFR2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND₅₀) is typically 10-50 ng/mL in the presence of 50 ng/mL Recombinant Human VEGFR2/KDR/Flk‑1 Fc Chimera and 10 ng/mL Recombinant Human VEGF₁₆₅.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

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Detection of VEGFR2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Neutralization

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VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. Recombinant Human VEGFR2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF₁₆₅(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅(10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND₅₀is typically 10-50 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGFR2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References

Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Entrez Gene IDs

3791 (Human); 16542 (Mouse)

Alternate Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway
Authors: T Tamari, R Kawar-Jara, O Doppelt, B Giladi, N Sabbah, H Zigdon-Gil
Cells, 2020;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Dual Action of Sulfated Hyaluronan on Angiogenic Processes in Relation to Vascular Endothelial Growth Factor-A
Authors: L Koehler, G Ruiz-Gómez, K Balamuruga, S Rother, J Freyse, S Möller, M Schnabelra, S Köhling, S Djordjevic, D Scharnwebe, J Rademann, MT Pisabarro, V Hintze
Sci Rep, 2019;9(1):18143.
Species: Human
Sample Types: Spheroid
Applications: Neutralization
Bone marrow characterization in COPD: a multi-level network analysis
Authors: N Toledo-Pon, G Noell, A Jahn, A Iglesias, MA Duran, J Iglesias, A Rios, S Scrimini, R Faner, O Gigirey, A Agustí, BG Cosío
Respir. Res., 2018;19(1):118.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
Authors: Gabriela Odent Grig
J Tissue Eng Regen Med, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of a Novel Pathogenic Germline KDR Variant in Melanoma
Authors: IP Silva, A Salhi, KM Giles, M Vogelsang, SW Han, N Ismaili, KP Lui, EM Robinson, MA Wilson, RL Shapiro, A Pavlick, J Zhong, T Kirchhoff, I Osman
Clin. Cancer Res., 2016;22(10):2377-85.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
PLoS ONE, 2014;9(4):e93965.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
MicroRNA-16 affects key functions of human endothelial progenitor cells.
Authors: Goretti E, Rolland-Turner M, Leonard F, Zhang L, Wagner D, Devaux Y
J Leukoc Biol, 2013;93(5):645-55.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature, 2011;475(7355):226-30.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
Leuk. Res., 2008;32(8):1244-58.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.
Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
Biochem. Biophys. Res. Commun., 2008;368(3):543-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007;177(3):489-500.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem Cells, 2007;25(2):490-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
Blood, 2007;109(4):1479-89.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Authors: Lin YL, Liang YC, Chiang BL
J. Leukoc. Biol., 2007;82(6):1473-80.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
Stem Cells, 2007;25(8):1862-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Thiol regulation of vascular endothelial growth factor-A and its receptors in human retinal pigment epithelial cells.
Authors: Sreekumar PG, Kannan R, de Silva AT, Burton R, Ryan SJ, Hinton DR
Biochem. Biophys. Res. Commun., 2006;346(4):1200-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry