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Human VEGFR2/KDR/Flk-1 Antibody

Catalog #: MAB3572
21 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB3572-SP
MAB3572-100
MAB3572-500
Detection of VEGFR2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (21)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human VEGFR2/KDR/Flk-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VEGFR2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3 or recombinant mouse VEGFR2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human VEGFR2/KDR/Flk-1
Ala20-Glu764
Accession # P35968
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize VEGFR2/KDR/Flk‑1-mediated inhibition of proliferation in HUVEC human umbilical vein endothelial cells. The Neutralization Dose (ND50) is typically 10-50 ng/mL in the presence of 50 ng/mL Recombinant Human VEGFR2/KDR/Flk‑1 Fc Chimera and 10 ng/mL Recombinant Human VEGF165.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VEGFR2/KDR/Flk-1 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of VEGFR2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Neutralization VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. View Larger

VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. Recombinant Human VEGFR2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF165(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165(10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND50 is typically 10-50 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR2/KDR/Flk-1

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGFR2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.

References
  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2

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Citations for Human VEGFR2/KDR/Flk-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells
    Authors: SA Luff, JP Creamer, S Valsoni, C Dege, R Scarfò, A Dacunto, S Cascione, LN Randolph, E Cavalca, I Merelli, SA Morris, A Ditadi, CM Sturgeon
    Nature Cell Biology, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Therapeutic correction of hemophilia A by transplantation of hPSC-derived liver sinusoidal endothelial cell progenitors
    Authors: BK Gage, S Merlin, C Olgasi, A Follenzi, GM Keller
    Cell Reports, 2022;39(1):110621.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&alpha and VEGF expression
    Authors: D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen
    Mol Med Rep, 2021;23(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells
    Authors: E Gambini, I Martinelli, I Stadiotti, MC Vinci, A Scopece, L Eramo, E Sommariva, J Resta, S Benaouadi, E Cogliati, A Paolin, A Parini, G Pompilio, F Savagner
    Int J Mol Sci, 2020;21(20):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway
    Authors: T Tamari, R Kawar-Jara, O Doppelt, B Giladi, N Sabbah, H Zigdon-Gil
    Cells, 2020;9(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Dual Action of Sulfated Hyaluronan on Angiogenic Processes in Relation to Vascular Endothelial Growth Factor-A
    Authors: L Koehler, G Ruiz-Gómez, K Balamuruga, S Rother, J Freyse, S Möller, M Schnabelra, S Köhling, S Djordjevic, D Scharnwebe, J Rademann, MT Pisabarro, V Hintze
    Sci Rep, 2019;9(1):18143.
    Species: Human
    Sample Types: Spheroid
    Applications: Neutralization
  7. Bone marrow characterization in COPD: a multi-level network analysis
    Authors: N Toledo-Pon, G Noell, A Jahn, A Iglesias, MA Duran, J Iglesias, A Rios, S Scrimini, R Faner, O Gigirey, A Agustí, BG Cosío
    Respir. Res., 2018;19(1):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
    Authors: Gabriela Odent Grig
    J Tissue Eng Regen Med, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Identification of a Novel Pathogenic Germline KDR Variant in Melanoma
    Authors: IP Silva, A Salhi, KM Giles, M Vogelsang, SW Han, N Ismaili, KP Lui, EM Robinson, MA Wilson, RL Shapiro, A Pavlick, J Zhong, T Kirchhoff, I Osman
    Clin. Cancer Res., 2016;22(10):2377-85.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
    Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
    PLoS ONE, 2014;9(4):e93965.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. MicroRNA-16 affects key functions of human endothelial progenitor cells.
    Authors: Goretti E, Rolland-Turner M, Leonard F, Zhang L, Wagner D, Devaux Y
    J Leukoc Biol, 2013;93(5):645-55.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011;475(7355):226-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
    Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
    Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.
    Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
    Biochem. Biophys. Res. Commun., 2008;368(3):543-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
    Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
    Leuk. Res., 2008;32(8):1244-58.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
    Authors: Ball SG, Shuttleworth CA, Kielty CM
    J. Cell Biol., 2007;177(3):489-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
    Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
    Blood, 2007;109(4):1479-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007;82(6):1473-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
    Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
    Stem Cells, 2007;25(8):1862-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Thiol regulation of vascular endothelial growth factor-A and its receptors in human retinal pigment epithelial cells.
    Authors: Sreekumar PG, Kannan R, de Silva AT, Burton R, Ryan SJ, Hinton DR
    Biochem. Biophys. Res. Commun., 2006;346(4):1200-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human VEGF R2/KDR/Flk-1 Antibody
By Anonymous on 08/19/2016
Application: ELISA Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human
ELISA Human VEGF R2/KDR/Flk-1 Antibody MAB3572