Human VEGFR2/KDR/Flk-1 Antibody Summary
Ala20-Glu764
Accession # P35968
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of VEGFR2/KDR/Flk‑1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. Recombinant Human VEGFR2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF165(Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165(10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND50 is typically 10-50 ng/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VEGFR2/KDR/Flk-1
VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. Mature VEGFR2 is composed of a 745 aa extracellular domain, a 25 aa transmembrane domain and a 567 aa cytoplasmic domain. In contrast to VEGFR1 which binds both PlGF and VEGF with high affinity, VEGFR2 binds VEGF but not PlGF with high affinity. The recombinant soluble VEGFR2/Fc chimera binds VEGF with high affinity and is a potent VEGF antagonist.
Product Datasheets
Citations for Human VEGFR2/KDR/Flk-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
21
Citations: Showing 1 - 10
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Identification of a retinoic acid-dependent haemogenic endothelial progenitor from human pluripotent stem cells
Authors: SA Luff, JP Creamer, S Valsoni, C Dege, R Scarfò, A Dacunto, S Cascione, LN Randolph, E Cavalca, I Merelli, SA Morris, A Ditadi, CM Sturgeon
Nature Cell Biology, 2022;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Therapeutic correction of hemophilia A by transplantation of hPSC-derived liver sinusoidal endothelial cell progenitors
Authors: BK Gage, S Merlin, C Olgasi, A Follenzi, GM Keller
Cell Reports, 2022;39(1):110621.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hyperoxia sensitizes hypoxic HeLa cells to ionizing radiation by downregulating HIF?1&alpha and VEGF expression
Authors: D Dong, Y Fu, F Chen, J Zhang, H Jia, J Li, H Wang, J Wen
Mol Med Rep, 2021;23(1):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Differences in Mitochondrial Membrane Potential Identify Distinct Populations of Human Cardiac Mesenchymal Progenitor Cells
Authors: E Gambini, I Martinelli, I Stadiotti, MC Vinci, A Scopece, L Eramo, E Sommariva, J Resta, S Benaouadi, E Cogliati, A Paolin, A Parini, G Pompilio, F Savagner
Int J Mol Sci, 2020;21(20):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Paracrine Role of Endothelial Cells in Bone Formation via CXCR4/SDF-1 Pathway
Authors: T Tamari, R Kawar-Jara, O Doppelt, B Giladi, N Sabbah, H Zigdon-Gil
Cells, 2020;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dual Action of Sulfated Hyaluronan on Angiogenic Processes in Relation to Vascular Endothelial Growth Factor-A
Authors: L Koehler, G Ruiz-Gómez, K Balamuruga, S Rother, J Freyse, S Möller, M Schnabelra, S Köhling, S Djordjevic, D Scharnwebe, J Rademann, MT Pisabarro, V Hintze
Sci Rep, 2019;9(1):18143.
Species: Human
Sample Types: Spheroid
Applications: Neutralization -
Bone marrow characterization in COPD: a multi-level network analysis
Authors: N Toledo-Pon, G Noell, A Jahn, A Iglesias, MA Duran, J Iglesias, A Rios, S Scrimini, R Faner, O Gigirey, A Agustí, BG Cosío
Respir. Res., 2018;19(1):118.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells
Authors: Gabriela Odent Grig
J Tissue Eng Regen Med, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of a Novel Pathogenic Germline KDR Variant in Melanoma
Authors: IP Silva, A Salhi, KM Giles, M Vogelsang, SW Han, N Ismaili, KP Lui, EM Robinson, MA Wilson, RL Shapiro, A Pavlick, J Zhong, T Kirchhoff, I Osman
Clin. Cancer Res., 2016;22(10):2377-85.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.
Authors: Jarajapu Y, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter S, Raizada M, Boulton M, Grant M
PLoS ONE, 2014;9(4):e93965.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
MicroRNA-16 affects key functions of human endothelial progenitor cells.
Authors: Goretti E, Rolland-Turner M, Leonard F, Zhang L, Wagner D, Devaux Y
J Leukoc Biol, 2013;93(5):645-55.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature, 2011;475(7355):226-30.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.
Authors: Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
Invest. Ophthalmol. Vis. Sci., 2010;51(2):1190-7.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.
Authors: Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
Biochem. Biophys. Res. Commun., 2008;368(3):543-9.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.
Authors: Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foa R, Peschle C, Sporn M, Testa U
Leuk. Res., 2008;32(8):1244-58.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007;177(3):489-500.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
Authors: Rajesh</LastName><ForeNam D</Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem Cells, 2007;25(2):490-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.
Authors: Caldas H, Fangusaro JR, Boue DR, Holloway MP, Altura RA
Blood, 2007;109(4):1479-89.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
Authors: Lin YL, Liang YC, Chiang BL
J. Leukoc. Biol., 2007;82(6):1473-80.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.
Authors: Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foa R, Peschle C, Testa U
Stem Cells, 2007;25(8):1862-71.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Thiol regulation of vascular endothelial growth factor-A and its receptors in human retinal pigment epithelial cells.
Authors: Sreekumar PG, Kannan R, de Silva AT, Burton R, Ryan SJ, Hinton DR
Biochem. Biophys. Res. Commun., 2006;346(4):1200-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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