Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 576721
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Product Specifications

Immunogen

KLH-coupled human VIP synthetic peptides HSDAVFTDNYTR and KYLNSILN

Specificity

Detects recombinant human VIP in direct ELISAs. It does not detect the C-terminal region (aa 145-152).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VIP Antibody

VIP antibody in Human Stomach by Immunohistochemistry (IHC-P).

VIP in Human Stomach.

VIP was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human VIP Monoclonal Antibody (Catalog # MAB6079) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human VIP Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human stomach and human stomach cancer tissue

Reviewed Applications

Read 1 review rated 5 using MAB6079 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VIP

Vasoactive intestinal peptide (VIP) is a 28 amino acid (aa) peptide that exerts wide-ranging effects in embryonic and adult tissues through interactions with the 7‑transmembrane spanning receptors VIP R1 and VIP R2. The VIP proprotein is proteolytically processed to release bioactive VIP as well as the bioactive peptides PHV and PHM. VIP regulates appetite and feeding behavior and stimulates insulin and glucagon secretion in pancreatic islet cells. It also regulates astrocyte function, multiple aspects of cardiac function, and TLR function in innate immune responses. Mature VIP shares 100% aa sequence identity with mouse and rat VIP.

Long Name

Vasoactive Intestinal Peptide

Alternate Names

MGC13587

Entrez Gene IDs

7432 (Human)

Gene Symbol

VIP

Additional VIP Products

Product Documents for Human VIP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VIP Antibody

For research use only

Citations for Human VIP Antibody

Customer Reviews for Human VIP Antibody (1)

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  • Human VIP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Stomach tissue
    Species: Human
    Verified Customer | Posted 02/21/2022
    Human VIP Antibody MAB6079

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Protocols

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FAQs

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