Human VRK1 Antibody

Catalog # Availability Size / Price Qty
MAB5835
MAB5835-SP
Human VRK1 Antibody in Data
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Human VRK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human VRK1 in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 1F6
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human VRK1
Met1-Lys396
Accession # Q99986
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human VRK1 antibody by Western Blot. View Larger

Detection of Human VRK1 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VRK1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry VRK1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

VRK1 in HeLa Human Cell Line. VRK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry VRK1 antibody in Human Testis by Immunohistochemistry (IHC-P). View Larger

VRK1 in Human Testis. VRK1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VRK1

Vaccinia-related kinase 1 (VRK1; also serine/threonine-protein kinase VRK1) is a 45 kDa member of the protein kinase superfamily, the CK1 Serine/Threonine protein kinase family, and the VRK subfamily. Human VRK1 is 396 amino acids (aa) in length, and contains one protein kinase domain (aa 37‑317). Human VRK1 shares 91% and 78% aa sequence identity with bovine and mouse VRK1, respectively. VRK1 is widely expressed with high expression in fetal liver, testis and thymus. VRK1 functions as a serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.

Long Name
Vaccinia-related Kinase 1
Entrez Gene IDs
7443 (Human); 22367 (Mouse)
Alternate Names
EC 2.7.11.1; MGC117401; MGC138280; MGC142070; PCH1; serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1; Vaccinia-related kinase 1; VRK1

Product Datasheets

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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