Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F6
View all VRK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VRK1
Met1-Lys396
Accession # Q99986

Specificity

Detects human VRK1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human VRK1 Antibody

Detection of Human VRK1 antibody by Western Blot.

Detection of Human VRK1 by Western Blot.

Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VRK1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VRK1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

VRK1 in HeLa Human Cell Line.

VRK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
VRK1 antibody in Human Testis by Immunohistochemistry (IHC-P).

VRK1 in Human Testis.

VRK1 was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human VRK1 Monoclonal Antibody (Catalog # MAB5835) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei of sperm cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human VRK1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis

Western Blot

1 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VRK1

Vaccinia-related kinase 1 (VRK1; also serine/threonine-protein kinase VRK1) is a 45 kDa member of the protein kinase superfamily, the CK1 Serine/Threonine protein kinase family, and the VRK subfamily. Human VRK1 is 396 amino acids (aa) in length, and contains one protein kinase domain (aa 37‑317). Human VRK1 shares 91% and 78% aa sequence identity with bovine and mouse VRK1, respectively. VRK1 is widely expressed with high expression in fetal liver, testis and thymus. VRK1 functions as a serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2.

Long Name

Vaccinia-related Kinase 1

Alternate Names

EC 2.7.11.1, MGC117401, MGC138280, MGC142070, PCH1, serine/threonine-protein kinase VRK1, vaccinia related kinase 1, vaccinia virus B1R-related kinase 1, Vaccinia-related kinase 1

Entrez Gene IDs

7443 (Human); 22367 (Mouse)

Gene Symbol

VRK1

UniProt

Q99986

Additional VRK1 Products

Product Documents for Human VRK1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human VRK1 Antibody

For research use only

Related Research Areas

Intracellular Kinases
p53 Pathway

Citations for Human VRK1 Antibody

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Protocols

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