Human VSIG4 Antibody

Siglec-7 protein binding to VSIG4-transfected Human Cell Line is Blocked by VSIG4 Antibody. In a functional flow cytometry test.

Recombinant Human Siglec-7/Fc protein (1138-SL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human VSIG4 and eGFP. (A) Binding is completely blocked by 50 µg/mL of Mouse Anti-Human VSIG4 Monoclonal Antibody (Catalog # MAB46462) but not by (B) Mouse IgG2A Isotype Control (MAB003). Protein binding was detected with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (FAB110A). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Detection of Human VSIG4 by Immunocytochemistry/ Immunofluorescence

VSIG4 blockade impairs C3b induced macrophages polarization. A Uniform manifold approximation and projection (UMAP) of single-cell RNA-seq from human decidual immune cells isolated from patients with RSA and healthy controls. The single-cell RNA sequencing data used in this study were previously published by Guo [9]. B Feature plot showed ICAM1 and VSIG4 expression in the different clusters between Ctrl vs. RSA as defined in (A). C Gene expression levels of VSIG4 and ICAM1 in decidual macrophages from normal pregnancies and RSA patients. D CLSM images showing the expression of VSIG4 protein in decidual macrophages from normal pregnancies and RSA patients. Scale bars = 5 μm. E Statistical histogram of VSIG4 mean fluorescence intensity of each decidual macrophages from normal pregnancies (n = 3) and RSA patients (n = 3). F Gene expression levels of IL1B, IL6 and TNF in macrophages with or without the addition of VSIG4 blocking antibody during co-culture with dNK. G Representative mean fluorescence intensity of CD80 (upper panel) or CD206 (lower panel) in macrophages with or without the addition of VSIG4 blocking antibody during co-culture with dNK. Experiments were repeated for 3 independent times. P values have been determined by two-tailed paired t-test Image collected and cropped by CiteAb from the following open publication (https://translational-medicine.biomedcentral.com/articles/10.1186/s1296…), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human VSIG4 by Immunocytochemistry/ Immunofluorescence

dNK cells from normal pregnancy express the natural ligand of VSIG4. A Gene expression levels of VSIG4 in peripheral blood NK cells (pNK), peripheral blood monocytes (pM), decidual macrophages (dM), and decidual NK cells (dNK). B Fluorescence image depicting the expression of VSIG4 protein on decidual macrophages. C Biological processes that are significantly enriched in Sankey dot pathway enrichment analysis of the upregulated genes of dNK relative to pNK. D Heat map of differential genes in complement and coagulation cascade pathways, n = 3 per group. E Gene set enrichment analysis (GSEA) revealed an increase in complement and coagulation cascade pathways (enrichment plot: COMPLEMENT AND COAGULATION CASCADES PATHWAYS, HSA04610) in dNK cells compared with pNK cells, n = 3 per group. F qPCR validation of the differential expression of the C3 gene in dNK cells and pNK cells. G CLSM images showing the secretion of C3b in pNKs and dNKs. The images were captured using the same parameters. Scale bars = 5 μm. H Statistical histogram of C3b mean fluorescence intensity of each decidual or peripheral NK cell. P values have been determined by two-tailed unpaired t-test Image collected and cropped by CiteAb from the following open publication (https://translational-medicine.biomedcentral.com/articles/10.1186/s1296…), licensed under a CC-BY license. Not internally tested by R&D Systems.