Human ZBTB38 Antibody AF5784: R&D Systems

Human ZBTB38 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human ZBTB38 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human ZBTB38
    Met1-Tyr232
    Accession # Q8NAP3
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ZBTB38 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line. PVDF membrane was probed with 1 µg/mL of Human ZBTB38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5784) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ZBTB38 at approximately 175 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZBTB38
ZBTB38 (Zinc finger and BTB domain-containing protein 38; also CIBZ and Zenon) is an early member of a family of Kaiso-like DNA binding proteins. Although its predicted MW is 134 kDa, it runs anomalously in SDS-PAGE at 175 kDa. ZBTB38 binds to singly methylated CpG (cytosine-phosphate-guanine) motifs. It is considered a transcriptional repressor that either homodimerizes, or heterodimerizes with ZBTB4, and subsequently interacts with either CtBP-1 or -2 to mediate repression. Human ZBTB38 is 1195 amino acids (aa) in length. It contains a BTB domain (aa 33‑100), an RD2 region (aa 158‑341) and ten C2H2-type zinc finger domains (aa 342‑1147). There is one potential alternate start site at Met595. Over aa 1‑232, human ZBTB38 shares 96% aa identity with mouse ZBTB38.
  • Long Name:
    Zinc Finger and BTB Domain-Containing Protein 38
  • Entrez Gene IDs:
    253461 (Human); 245007 (Mouse); 315936 (Rat)
  • Alternate Names:
    CIBZ; FLJ22332; FLJ31131; FLJ35036; STQTL10; ZBTB38; zinc finger and BTB domain containing 38; zinc finger and BTB domain-containing protein 38; ZNF921
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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