Human ZNF10 Antibody Summary
Accession # P21506
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human ZNF10 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line, HeLa human cervical epithelial carcinoma cell line, human kidney tissue, and human thymus tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human ZNF10 Monoclonal Antibody (Catalog # MAB9656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF10 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human Zinc finger protein ZNF10 (or KOX1) is a 573 amino acids protein encoded by the ZNF10 gene. ZNF10 contains a C2H2 zinc finger, and has been shown to function as a transcriptional repressor via the Kruppel-associated box (KRAB) domain of the protein. ZNF10 has been shown to interact with the nuclear hub protein TRIM28 to mediate chromatin-dependent processes like transcriptional repression, imprinting or suppression of endogenous retroviruses, representing a potential intrinsic antiretroviral defense.
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