IL-6 Antibody (OTI3G9)

Novus Biologicals | Catalog # NBP1-47810

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G9
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.

Specificity

This antibody is specific for Homo sapiens interleukin 6 (interferon, beta 2) (IL6).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse IL-6 Antibody (OTI3G9) (NBP1-47810) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-IL-6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for IL-6 Antibody (OTI3G9)

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810]

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810]

Western Blot: IL6 Antibody (3G9) [NBP1-47810] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 IL6 antibody (2G3) at 1:2000
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (3G9) [NBP1-47810] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IL6.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human prostate tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human breast tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human colon tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810]

Immunohistochemistry-Paraffin: IL-6 Antibody (3G9) [NBP1-47810] - Staining of paraffin-embedded Human Kidney tissue using anti-IL6 mouse monoclonal antibody.
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)
IL-6 Antibody (OTI3G9)

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Western Blot: IL-6 Antibody (OTI3G9) [NBP1-47810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6.
IL-6 Antibody (OTI3G9)

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] -

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) [NBP1-47810] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.)

Applications for IL-6 Antibody (OTI3G9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 5 using NBP1-47810 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IL-6

IL6 is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA (IL6R; MIM 147880), and the shared signaling receptor gp130 (IL6ST; MIM 600694)

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 3569 (Human)

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for IL-6 Antibody (OTI3G9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for IL-6 Antibody (OTI3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-6 Antibody (OTI3G9)

Customer Reviews for IL-6 Antibody (OTI3G9) (1)

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Customer Images

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  • Name: Hayley Derricott
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 09/03/2014
    IHC-P anti-IL-6 Tonsil
    IL-6 Antibody (OTI3G9) NBP1-47810

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 Antibody (OTI3G9)

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    • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
      A: We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
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