IL-6 Antibody (OTI3G9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71027

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G9

Format

Azide and BSA Free
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 29-212 of human IL-6 (NP_000591) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

23.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IL-6 Antibody (OTI3G9) - Azide and BSA Free

Western Blot: IL-6 Antibody (OTI3G9)Azide and BSA Free [NBP2-71027]

Western Blot: IL-6 Antibody (OTI3G9)Azide and BSA Free [NBP2-71027]

Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - IL6 antibody (3G9) at 1:500000 + recombinant human IL6 antibody (2G3) at 1:2000
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Human endometrium tissue using anti-IL6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IL6 mouse monoclonal antibody.
Western Blot: IL-6 Antibody (OTI3G9)Azide and BSA Free [NBP2-71027]

Western Blot: IL-6 Antibody (OTI3G9)Azide and BSA Free [NBP2-71027]

Western Blot: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL-6 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL-6. Positive lysates LY424617 (100ug) and LC424617 (20ug).
Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunocytochemistry/ Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunocytochemistry/Immunofluorescence: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Anti-IL-6 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IL-6.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human breast tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human breast tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human colon tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human colon tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human Kidney tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Carcinoma of Human kidney tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human lung tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Carcinoma of Human lung tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human Ovary tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Adenocarcinoma of Human ovary tissue using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human pancreas tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.
Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027]

Immunohistochemistry-Paraffin: IL-6 Antibody (OTI3G9) - Azide and BSA Free [NBP2-71027] - Staining of Human prostate tissue within the normal limits using anti-IL-6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Applications for IL-6 Antibody (OTI3G9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:50000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Gene Symbol

IL6

Additional IL-6 Products

Product Documents for IL-6 Antibody (OTI3G9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-6 Antibody (OTI3G9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 Antibody (OTI3G9) - Azide and BSA Free

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  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A:

    We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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