IL-6 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-77894

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Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all IL-6 Primary Antibodies »

Product Specifications

Immunogen

IL-6 Antibody prepared from rabbit antiserum after repeated immunizations with recombinant mouse IL-6 protein produced in E.coli. (Uniprot: P08505)

Reactivity Notes

Cross-reactivity with IL-6 from other sources has not been determined

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above

Store this antibody at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for IL-6 Antibody - Azide and BSA Free

Western Blot: IL-6 Antibody [NBP1-77894]

Western Blot: IL-6 Antibody [NBP1-77894]

Western Blot: IL-6 Antibody [NBP1-77894] - Shows detection of recombinant mouse IL-6 raised in E.coli. Recombinant truncated protein (0.1 ug, 21.7 kDa) was loaded on to an SDS-PAGE gel, and after separation, transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse IL-6 antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody DyLight (TM) 649 conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer for 30 min. at RT.
Immunohistochemistry: IL-6 Antibody [NBP1-77894]

Immunohistochemistry: IL-6 Antibody [NBP1-77894]

Immunohistochemistry: IL-6 Antibody [NBP1-77894] - Shows nuclear positivity of islets of Langerhans (brown staining) and cytoplasmic staining in mouse pancreas at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
IL-6 Antibody

IL-6 Antibody

Immunohistochemistry with anti-IL-6 antibody showing cytoplasmic IL-6 staining in mouse pancreas exocrine and endocrine sections with islets of Langerhans at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit anti-mouse IL-6 antibody at 1:50 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3'3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.
IL-6 Antibody - Azide and BSA Free

Flow Cytometry: IL-6 Antibody - Azide and BSA Free [NBP1-77894] -

Flow Cytometry Results of Rabbit Anti-Mouse IL6 Antibody in human THP-1 cell line. The orange histogram represents the THP-1 cells that were activated with 100ng/mL LPS for 24 hours. The green histogram are untreated THP-1 cells. These two populations were intracellularly stained for 30 minutes at 4°C in 1× BD Perm/Wash™ buffer. The primary stain was a 1:100 dilution of the Anti-Mouse IL-6 (RABBIT) Polyclonal Antibody and the secondary stain was the Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated. The secondary stain was for 30 minutes at 4°C and was kept protected from light. The blue histogram is the THP-1 cells that were untreated and only stained with the secondary antibody. The red histogram is the untreated THP-1 cells that were not stained. Prior to staining, the cells for all conditions were permeabilized with BD Fixation/Permeabilization ™ solution for 20 minutes at 4°C. All washes and stains were performed in the BD 1× Perm/Wash™ buffer.
IL-6 Antibody - Azide and BSA Free

Flow Cytometry: IL-6 Antibody - Azide and BSA Free [NBP1-77894] -

Flow Cytometry Results of Rabbit Anti-Mouse IL6 Antibody in mouse Raw 264.7 cell line. The orange histogram represents the Raw 264.7 murine cells that were activated with 100ng/mL LPS for 24 hours. The green histogram are untreated Raw 264.7 cells. These two populations were intracellularly stained for 30 minutes at 4°C in 1× BD Perm/Wash™ buffer. The primary stain was a 1:30 dilution of the Anti-Mouse IL-6 (RABBIT) Polyclonal Antibody and the secondary stain was the Anti-RABBIT IgG (H&L) (GOAT) Antibody Fluorescein Conjugated. The secondary stain was for 30 minutes at 4°C and was kept protected from light. The blue histogram is the Raw 264.7 murine cells that were untreated and only stained with the secondary antibody. The red histogram is the untreated Raw 264.7 murine cells that were not stained. Prior to staining, the cells for all conditions were permeabilized with BD Fixation/Permeabilization ™ solution for 20 minutes at 4°C. All washes and stains were performed in the BD 1× Perm/Wash™ buffer.

Applications for IL-6 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
This purified antibody has been tested for use in Immunohistochemistry and western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21.7 kDa in size corresponding to the mature 187 amino acid mouse IL-6 protein by western blotting in appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6

IL-6 is a multifunctional cytokine that has an important role in host defense by regulating immune and inflammatory responses. It is expressed by a variety of normal and transformed cells including T cells, B cells, monocytes/macrophages, fibroblasts and various tumor cells. IL-6 has diverse biological functions, it stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Recombinant human IL-6 is a 20.9 kDa protein containing 184 amino acid residues. Human IL-6 is active on murine cells but murine IL-6 is inactive on human cells.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

16193 (Mouse); 16193 (Mouse)

Gene Symbol

IL6

UniProt

P08505

Additional IL-6 Products

Product Documents for IL-6 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for IL-6 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for IL-6 Antibody - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-6 Antibody - Azide and BSA Free

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  • Q: I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?

    A:

    We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.

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