Light & Electron Microscope-based Kits

Immunohistochemistry kits designed to detect apoptosis in fixed cells, embedded tissue, and frozen tissue using light and electron microscopy.

DNA fragmentation represents a characteristic hallmark of apoptosis. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is an established method for detecting DNA fragments.

TACS® TdT in situ DAB

Detection of Apoptosis in Post-weaning Mouse Breast Tissue using TACS TdT DAB Kit
Post-weaning mouse breast tissue was fixed in 10% neutral buffered formalin, embedded in paraffin, and sectioned at five microns. Deparaffinized and rehydrated sections were subsequently processed using the TACS TdT DAB Kit protocol (Catalog # 4810-30-K) and visualized by light microscopy.

The TACS TdT kits contain a highly purified form of the TdT enzyme for the enzymatic incorporation of biotinylated nucleotides. Biotin labeling is achieved using streptavidin-horseradish peroxidase and colorimetric substrates diaminobenzidine (DAB) or TACS Blue Label™.

Kits contain Streptavidin-HRP, DAB, Methyl Green counterstain, and reagents necessary for generating positive controls.

Label: Biotin/Streptavidin-HRP/DAB
Testing Format: Light Microscopy, Electron Microscopy
Sample Type: Fixed cells, Embedded tissues, Frozen tissues
Size: 30 Tests (Catalog # 4810-30-K)

TdT In Situ Apoptosis Detection Kit - Core Reagents (Catalog # 4810-30-CK)
TACS TdT in situ Replenisher Kit -replace limiting reagents (Catalog # 4810-30-R)

TACS TdT in situ TACS Blue Label

Kits contain Streptavidin-HRP, TACS BlueLabel, Nuclear Fast Red counterstain, and reagents necessary for generating positive controls.

Label: Biotin/Streptavidin-HRP/TACS Blue Label
Testing Format: Light Microscopy, Electron Microscopy
Sample Type: Fixed cells, Embedded tissues, Frozen tissues
Size: 30 Tests (Catalog # 4811-30-K)

TACS TdT in situ Replenisher Kit -replace limiting reagents (Catalog # 4810-30-R)

TACS XL Basic

TACS XL kits embody a novel approach for the detection of apoptosis.  This assay is based on incorporation of bromodeoxyuridine (BrdU) at the 3’ OH ends of the DNA fragments that form during apoptosis.  This detection system utilizes a biotin conjugated anti-BrdU antibody and streptavidin-horseradish peroxidase. 

Kits contain all core reagents necessary for the TUNEL reaction with exception of the visualizing label.

Label: BrdU/conjugated BrdU Antibody/User supplied label
Testing Format: Light Microscopy, Electron Microscopy
Sample Type: Fixed cells, Embedded tissues, Frozen tissues
Size: 30 Tests (Catalog # 4828-30-K)

TACS XL Replenisher Kit -replace limiting reagents (Catalog # 4828-30-R)

TACS XL DAB

Kits contain biotin-conjugated anti-BrdU, Streptavidin-HRP, DAB, Methyl Green counterstain, and reagents necessary for generating positive controls.

Label: BrdU/conjugated BrdU Antibody/Streptavidin-HRP/DAB
Testing Format: Light Microscopy, Electron Microscopy
Sample Type: Fixed cells, Embedded tissues, Frozen tissues
Size: 30 Tests (Catalog # 4828-30-DK)

TACS XL Replenisher Kit -replace limiting reagents (Catalog # 4828-30-R)

TACS XL TACS Blue Label

DNA Fragmentation in Mouse Lung Parenchyma Tissue Detected using the TACS XL® Blue Kit
Mouse lung parenchyma tissue was fixed and embedded in paraffin. Five micron sections were prepared and immobilized on slides. The tissue was labeled as describe in the TACSXL protocol (Catalog # 4828-30-BK) and analyzed by light microscopy for DNA fragmentation indicated by the blue stained cells. Data was provided courtesy of F. D'Agostini, University of Genova Via A. Pastore, Genova, Italy.

Kits contain biotin-conjugated anti-BrdU, Streptavidin-HRP, TACS BlueLabel, Nuclear Fast Red counterstain, and reagents necessary for generating positive controls.

Label: BrdU/Conjugated BrdU Antibody/Streptavidin-HRP/TACS Blue Label
Testing Format: Light Microscopy, Electron Microscopy
Sample Type: Fixed cells, Embedded tissues, Frozen tissues
Size: 30 Tests (Catalog # 4828-30-BK)

TACS XL Replenisher Kit -replace limiting reagents (Catalog # 4828-30-R)

Positive Control Slides

R&D Systems also offers additional slides of fixed apoptotic cell and tissue samples that can be used to further support positive results (Catalog # 4800-30-21, 4800-30-40, 4800-30-41).

TACS XL and TACS Blue are trademarks of Trevigen, Inc.