LTRIN Antibody

Catalog #: MAB10178 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10178-100
MAB10178-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

LTRIN Antibody Summary

Specificity

Detects LTRIN in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1009804

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic Aedes aegypti LTRIN peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB101781).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of LTRIN by Western Blot. Western blot shows lysates of Aedes aegypti LTRIN peptide. PVDF membrane was probed with 2 µg/mL of Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB10178) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for LTRIN at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ELISA

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LTRIN ELISA Standard Curve. LTRIN protein was serially diluted 2-fold and captured by Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB10178) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-LTRIN Monoclonal Antibody (Catalog # MAB101781) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LTRIN

Salivary gland protein LTRIN, a 15 Kilodalton protein from mosquito Aedes aegypti, has been shown to facilitate the transmission of Zika virus (ZIKV) probably by interfering with signaling through the lymphotoxin-beta receptor (LT beta R).

Long Name

Multiple Coagulation Factor Deficiency Protein 2

Entrez Gene IDs

23687673 (Insect)

Alternate Names

LTRIN